Transcript: Human XM_017023441.2

PREDICTED: Homo sapiens VAC14 component of PIKFYVE complex (VAC14), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
VAC14 (55697)
Length:
8363
CDS:
258..2180

Additional Resources:

NCBI RefSeq record:
XM_017023441.2
NBCI Gene record:
VAC14 (55697)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017023441.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000153770 CTCAATGACAAGCTGTACGAA pLKO.1 309 CDS 100% 3.000 4.200 N VAC14 n/a
2 TRCN0000343619 TTGTTGCGAGAGAGGATTTAC pLKO_005 792 CDS 100% 13.200 9.240 N VAC14 n/a
3 TRCN0000352951 CCATCCTACTGCAGACGTTAT pLKO_005 1591 CDS 100% 10.800 7.560 N VAC14 n/a
4 TRCN0000343678 TCCAGATCCTGGGTGACAATG pLKO_005 922 CDS 100% 10.800 7.560 N VAC14 n/a
5 TRCN0000153900 CAGTGTGAAGTTTGCTGAGAT pLKO.1 1013 CDS 100% 4.950 3.465 N VAC14 n/a
6 TRCN0000154006 CGTGCAAATCAAGCATGTGAT pLKO.1 395 CDS 100% 4.950 3.465 N VAC14 n/a
7 TRCN0000343676 CGTGCAAATCAAGCATGTGAT pLKO_005 395 CDS 100% 4.950 3.465 N VAC14 n/a
8 TRCN0000155198 GCTTCAATGATGCAGACAGCA pLKO.1 559 CDS 100% 2.640 1.848 N VAC14 n/a
9 TRCN0000173710 CCAGACATTAACCTGCTGGAT pLKO.1 873 CDS 100% 2.640 1.584 N Vac14 n/a
10 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 8313 3UTR 100% 4.950 2.475 Y ERN2 n/a
11 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 8313 3UTR 100% 4.950 2.475 Y P3H4 n/a
12 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 8313 3UTR 100% 4.950 2.475 Y P3H4 n/a
13 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 8315 3UTR 100% 4.950 2.475 Y CFLAR n/a
14 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 8315 3UTR 100% 4.950 2.475 Y C19orf31 n/a
15 TRCN0000157476 GCCAACATGGTGAAACCCAAT pLKO.1 7089 3UTR 100% 4.050 2.025 Y NFASC n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017023441.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03632 pDONR223 100% 79.1% 68.1% None (many diffs) n/a
2 ccsbBroad304_03632 pLX_304 0% 79.1% 68.1% V5 (many diffs) n/a
3 TRCN0000469365 CACACTCTTAGACTCCCATACTGT pLX_317 16.6% 79.1% 68.1% V5 (many diffs) n/a
4 ccsbBroadEn_12254 pDONR223 100% 46.3% 37% None (many diffs) n/a
5 ccsbBroad304_12254 pLX_304 0% 46.3% 37% V5 (many diffs) n/a
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