Construct: ORF ccsbBroad304_03751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006391.1_s304c1, BRDN0000410460
- Derived from:
- ccsbBroadEn_03751
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA4L2 (56901)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03751
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56901 | NDUFA4L2 | NDUFA4 mitochondrial comple... | NM_020142.3 | 100% | 100% | |
| 2 | mouse | 407790 | Ndufa4l2 | NADH dehydrogenase (ubiquin... | NM_001098789.1 | 88.8% | 94.2% | (many diffs) |
| 3 | mouse | 407790 | Ndufa4l2 | NADH dehydrogenase (ubiquin... | XM_006513834.3 | 75.3% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 327
- ORF length:
- 261
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc aggagccagt cttggggccc gcttctaccg gcagatcaaa agacatccgg 121 ggatcatccc gatgatcggc ttaatctgcc tgggcatggg cagcgctgcg ctttacttgc 181 tgcgactcgc ccttcgcagc cccgacgtct gctgggacag aaagaacaac ccggagccct 241 ggaaccgcct gagccccaat gaccaataca agttccttgc agtttccact gactataaga 301 agctgaagaa ggaccggcca gacttctacc caactttctt gtacaaagtg gttggtaagc 361 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 421 ggcgcgttaa gtcgacaatc aacctctgga tta