Construct: ORF ccsbBroad304_03766
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017879.1_s304c1, BRDN0000401306
- Derived from:
- ccsbBroadEn_03766
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC42SE2 (56990)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03766
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56990 | CDC42SE2 | CDC42 small effector 2 | NM_001038702.1 | 100% | 100% | |
| 2 | human | 56990 | CDC42SE2 | CDC42 small effector 2 | NM_020240.2 | 100% | 100% | |
| 3 | human | 56990 | CDC42SE2 | CDC42 small effector 2 | XM_017009648.2 | 100% | 100% | |
| 4 | human | 56990 | CDC42SE2 | CDC42 small effector 2 | XM_017009649.2 | 100% | 100% | |
| 5 | mouse | 72729 | Cdc42se2 | CDC42 small effector 2 | NM_178626.3 | 90.8% | 100% | (many diffs) |
| 6 | mouse | 72729 | Cdc42se2 | CDC42 small effector 2 | XM_006534320.3 | 90.8% | 100% | (many diffs) |
| 7 | mouse | 72729 | Cdc42se2 | CDC42 small effector 2 | XM_030246369.1 | 90.8% | 100% | (many diffs) |
| 8 | mouse | 72729 | Cdc42se2 | CDC42 small effector 2 | XM_030246370.1 | 90.8% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 321
- ORF length:
- 252
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgaattc tggttgtgtt tcaactgctg tattgcagaa cagcctcagc 121 ctaaaaggcg acggcggatt gacagaagta tgattggaga gcccacaaac tttgtgcata 181 cagctcatgt tggatcagga gacctgttca gtggaatgaa ttcagttagc tccattcaga 241 accaaatgca gtccaaggga ggttatggag gtggaatgcc tgccaatgtc cagatgcagc 301 tcgtggatac gaaggcggga ttgccaactt tcttgtacaa agtggttggt aagcctatcc 361 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 421 ttaagtcgac aatcaacctc tggatta