Construct: ORF ccsbBroad304_03786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002230.1_s304c1, BRDN0000407777
- Derived from:
- ccsbBroadEn_03786
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPPIN (57119)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03786
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57119 | EPPIN | epididymal peptidase inhibitor | NM_020398.4 | 100% | 100% | |
| 2 | human | 57119 | EPPIN | epididymal peptidase inhibitor | NM_001302861.2 | 90.2% | 58.6% | 222_232del;381_382ins29 |
| 3 | human | 100526773 | EPPIN-WFDC6 | EPPIN-WFDC6 readthrough | NM_001198986.2 | 74.1% | 72.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg atcttctgga cttttgagcc tcctggtgct attcgtcctc ttagcgaatg 121 tccagggacc tggtctgact gattggttat ttcccaggag atgtcccaaa atcagagaag 181 aatgtgaatt ccaagaaagg gatgtgtgta caaaggacag acaatgccag gacaacaaga 241 agtgttgtgt cttcagctgc ggaaaaaaat gtttagatct caaacaagat gtatgcgaaa 301 tgccaaaaga aactggcccc tgcctggctt attttcttca ttggtggtat gacaagaaag 361 ataatacttg ctccatgttt gtctatggtg gctgccaggg aaacaataac aacttccaat 421 ccaaagccaa ctgcctgaac acctgcaaga ataaacgctt tccctgccca actttcttgt 481 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 541 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a