Construct: ORF ccsbBroad304_03796
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007867.1_s304c1, BRDN0000404737
- Derived from:
- ccsbBroadEn_03796
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMIM8 (57150)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03796
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57150 | SMIM8 | small integral membrane pro... | NM_001042493.3 | 100% | 100% | |
| 2 | human | 57150 | SMIM8 | small integral membrane pro... | NM_020425.5 | 100% | 100% | |
| 3 | human | 57150 | SMIM8 | small integral membrane pro... | NM_001287445.2 | 64.4% | 43.8% | (many diffs) |
| 4 | mouse | 66291 | Smim8 | small integral membrane pro... | NM_001330755.1 | 85.5% | 88.6% | (many diffs) |
| 5 | mouse | 66291 | Smim8 | small integral membrane pro... | NM_001330756.1 | 85.5% | 88.6% | (many diffs) |
| 6 | mouse | 66291 | Smim8 | small integral membrane pro... | NM_001330757.1 | 85.5% | 88.6% | (many diffs) |
| 7 | mouse | 66291 | Smim8 | small integral membrane pro... | NM_025471.3 | 85.5% | 88.6% | (many diffs) |
| 8 | mouse | 66291 | Smim8 | small integral membrane pro... | XM_006538163.2 | 85.5% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 357
- ORF length:
- 291
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttcagcacct gagcctccaa cattcaaaaa ggaaccaccc aaagagaaag 121 agtttcaaag cccagggctc agaggggtgc gcacaacaac cttatttcgt gctgtgaatc 181 cagagctctt cattaaacct aacaaacctg taatggcttt cggattggta actctttcac 241 tttgcgtggc atatattggt tatctacatg caatacaaga gaataaaaag gacctctatg 301 aagctattga tagtgagggg cacagttata tgaggcggaa aacatccaaa tgggattacc 361 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 421 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 481 tta