Transcript: Mouse NM_001330756.1

Mus musculus small integral membrane protein 8 (Smim8), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Smim8 (66291)
Length:
794
CDS:
166..459

Additional Resources:

NCBI RefSeq record:
NM_001330756.1
NBCI Gene record:
Smim8 (66291)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001330756.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000127323 GCGCACACAACCACCTTATTT pLKO.1 247 CDS 100% 15.000 21.000 N Smim8 n/a
2 TRCN0000334558 GCGCACACAACCACCTTATTT pLKO_005 247 CDS 100% 15.000 21.000 N Smim8 n/a
3 TRCN0000348324 ATTGGTAACCCTTTCACTTTG pLKO_005 324 CDS 100% 10.800 15.120 N Smim8 n/a
4 TRCN0000127320 GCATCGGTACATGAGGAGAAA pLKO.1 420 CDS 100% 4.950 6.930 N Smim8 n/a
5 TRCN0000127319 GTGGTGAGTTTAGTCTCATTA pLKO.1 592 3UTR 100% 13.200 9.240 N Smim8 n/a
6 TRCN0000334560 GTGGTGAGTTTAGTCTCATTA pLKO_005 592 3UTR 100% 13.200 9.240 N Smim8 n/a
7 TRCN0000127322 GCTCTTCATTAAACCCAACAA pLKO.1 285 CDS 100% 4.950 3.465 N Smim8 n/a
8 TRCN0000334559 GCTCTTCATTAAACCCAACAA pLKO_005 285 CDS 100% 4.950 3.465 N Smim8 n/a
9 TRCN0000127321 CCTTTCACTTTGTGTGGCTTA pLKO.1 333 CDS 100% 4.050 2.835 N Smim8 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001330756.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03796 pDONR223 100% 85.5% 88.6% None (many diffs) n/a
2 ccsbBroad304_03796 pLX_304 0% 85.5% 88.6% V5 (many diffs) n/a
3 TRCN0000478528 GACATTGCCCATTAGATCCATACG pLX_317 100% 85.5% 88.6% V5 (many diffs) n/a
Download CSV