Construct: ORF ccsbBroad304_03816

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011311.1_s304c1, BRDN0000404054
Derived from:
ccsbBroadEn_03816
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPC25 (57405)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03816
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57405 SPC25 SPC25 component of NDC80 ki... NM_020675.4 100% 100%
2 human 57405 SPC25 SPC25 component of NDC80 ki... XM_011511516.2 82.4% 82.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
738
ORF length:
672
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt agaggacgaa ctggcacttt tcgataaaag cataaatgaa ttttggaata
121aattcaaaag tacggacacc tcctgtcaga tggcgggact aagagatacc tacaaggatt
181ccatcaaagc atttgcagaa aagctgtctg tgaaattaaa ggaagaagaa cgaatggttg
241agatgtttct ggaatatcaa aatcagatca gcaggcaaaa taagctcatt caagaaaaaa
301aggataactt gttaaaattg attgctgaag taaaaggcaa aaagcaggaa ttggaagtac
361tgactgcaaa tatccaggat cttaaggaag aatattctag gaagaaggaa actatttcta
421ctgctaataa agcgaatgca gagaggttga aaaggctgca gaaatctgca gacttgtata
481aagatcgact tggactagaa attcgaaaaa tttatggtga gaaattgcag tttattttca
541ctaatattga ccctaagaat cctgagagcc catttatgtt ttccttacat ctcaatgaag
601caagggacta tgaagtgtca gatagtgccc ctcatcttga gggcctagca gaatttcaag
661agaatgtaag gaagaccaac aatttttcag cttttcttgc caatgttcgg aaagctttta
721ctgccacggt ttataattac ccaactttct tgtacaaagt ggttggtaag cctatcccta
781accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
841agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)