Construct: ORF ccsbBroad304_03838
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000071.1_s304c1, BRDN0000400148
- Derived from:
- ccsbBroadEn_03838
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPB41L5 (57669)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03838
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | NM_001184938.4 | 100% | 100% | |
| 2 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | NM_001184939.3 | 100% | 100% | |
| 3 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | NM_001330307.2 | 85.7% | 85.7% | 0_1ins216 |
| 4 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | NM_001184937.2 | 69.8% | 64.8% | (many diffs) |
| 5 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | NM_001330310.2 | 65.6% | 60.9% | (many diffs) |
| 6 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | NM_020909.4 | 65.5% | 60.8% | (many diffs) |
| 7 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | XM_006712651.1 | 64.9% | 60.2% | (many diffs) |
| 8 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | XM_017004567.1 | 64.9% | 60.2% | (many diffs) |
| 9 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | XM_006712653.1 | 55.3% | 50.7% | (many diffs) |
| 10 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | NR_135920.3 | 25.2% | 1_142del;1478_1580del;1761_5997del | |
| 11 | human | 57669 | EPB41L5 | erythrocyte membrane protei... | NR_138472.2 | 25.1% | 1_142del;1480_1610del;1789_6025del | |
| 12 | mouse | 226352 | Epb41l5 | erythrocyte membrane protei... | NM_001113416.1 | 88.3% | 91.4% | (many diffs) |
| 13 | mouse | 226352 | Epb41l5 | erythrocyte membrane protei... | XM_006529400.1 | 88.3% | 91.4% | (many diffs) |
| 14 | mouse | 226352 | Epb41l5 | erythrocyte membrane protei... | XM_006529399.3 | 59.2% | 57.4% | (many diffs) |
| 15 | mouse | 226352 | Epb41l5 | erythrocyte membrane protei... | NM_145506.4 | 59.1% | 57.3% | (many diffs) |
| 16 | mouse | 226352 | Epb41l5 | erythrocyte membrane protei... | XM_006529397.1 | 59.1% | 57.3% | (many diffs) |
| 17 | mouse | 226352 | Epb41l5 | erythrocyte membrane protei... | XM_006529398.1 | 59.1% | 57.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1581
- ORF length:
- 1515
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gagtttcttc cgtagaacac tagggcgtcg gtctatgcgt aaacatgcag 121 agaaggaacg actccgagaa gcacaacgcg ccgccacaca tattcctgca gctggagatt 181 ctaagtccat catcacgtgt cgggtgtccc ttctggatgg tactgatgtt agtgtggact 241 tgccaaaaaa agccaaagga caagagttgt ttgatcagat tatgtaccac ctggacctga 301 ttgaaagcga ctattttggt ctgagattta tggattcagc acaagtagca cattggttgg 361 atggtacaaa aagcatcaaa aagcaagtaa aaattggttc accctattgt ctgcatcttc 421 gagttaagtt ttattcctca gaaccaaata accttcgtga ggagctaacc cggtatttat 481 ttgttcttca gttaaaacaa gatattctca gtggaaaatt agactgtccc tttgatacag 541 cagtgcaatt ggcagcttat aatctgcaag ctgaacttgg tgactatgat cttgctgagc 601 atagtcctga acttgtctca gagttcagat tcgtgcctat tcagactgaa gagatggaac 661 tggctatttt tgagaaatgg aaggaataca gaggtcaaac accagcacag gctgaaacca 721 attatctgaa taaagccaaa tggctagaaa tgtatggggt tgatatgcat gtggtcaagg 781 ctagagatgg gaatgactat agtttgggac taacaccaac aggagtcctt gtttttgaag 841 gagataccaa aattggctta tttttttggc cgaagataac cagattggat tttaagaaga 901 ataaattaac cttggtggtt gtagaagatg atgatcaggg caaagaacag gaacatacat 961 ttgtctttag actggatcat ccaaaagcat gcaaacattt atggaaatgt gctgtggagc 1021 atcatgcttt cttccgcctt cgaggccccg tccaaaagag ttctcatcga tcaggattta 1081 ttcgactagg atcacgattt agatatagtg ggaaaacaga gtatcagacc acaaaaacca 1141 ataaagcaag aagatcaaca tcctttgaaa gaaggcccag caaacgatat tctagacgaa 1201 ctctacaaat gaaagcatgt gctacaaaac ctgaagaact tagtgttcac aataatgttt 1261 cgacccaaag taatggctcc caacaggctt gggggatgag atctgctctg cctgtgagtc 1321 cttccatttc ctctgctcct gtgccagtgg agatagagaa tcttccacag agtcctggaa 1381 cagaccagca tgacaggaaa tggctctctg ctgccagcga ctgctgtcaa cgtggtggaa 1441 accagtggaa cacaagggcc ttgcccccac cccagaccgc acatagaaac tacactgact 1501 ttgttcatga gcacaatgtg aagaatgcag gaatccgtca tgatgttcat tttcctggcc 1561 atacagccat gactgagata tgcccaactt tcttgtacaa agtggttggt aagcctatcc 1621 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1681 ttaagtcgac aatcaacctc tggatta