Construct: ORF ccsbBroad304_03846
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002117.1_s304c1, BRDN0000408004
- Derived from:
- ccsbBroadEn_03846
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP4R4 (57718)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03846
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | NM_058237.2 | 100% | 100% | |
| 2 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | NM_001348142.1 | 90.7% | 90.7% | 0_1ins243 |
| 3 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | XM_024449672.1 | 90.7% | 90.7% | 0_1ins243 |
| 4 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | XM_017021528.2 | 89.4% | 89% | (many diffs) |
| 5 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | NM_001348143.2 | 87.7% | 87.7% | 0_1ins321 |
| 6 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | NM_001348144.2 | 87.7% | 87.7% | 0_1ins321 |
| 7 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | XM_011537039.2 | 87.7% | 87.7% | 0_1ins321 |
| 8 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | XM_011537040.2 | 87.7% | 87.7% | 0_1ins321 |
| 9 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | XM_024449673.1 | 87.7% | 87.7% | 0_1ins321 |
| 10 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | NR_145441.2 | 67.4% | 1_144del;2155_2180del;2790_3883del | |
| 11 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | NM_001348145.1 | 15.9% | 11.4% | (many diffs) |
| 12 | human | 57718 | PPP4R4 | protein phosphatase 4 regul... | NM_020958.2 | 13.2% | 12% | (many diffs) |
| 13 | mouse | 74521 | Ppp4r4 | protein phosphatase 4, regu... | NM_028980.3 | 88.4% | 90.8% | (many diffs) |
| 14 | mouse | 74521 | Ppp4r4 | protein phosphatase 4, regu... | XM_006516308.1 | 87.9% | 90.3% | (many diffs) |
| 15 | mouse | 74521 | Ppp4r4 | protein phosphatase 4, regu... | XM_011244194.2 | 80.1% | 79.3% | (many diffs) |
| 16 | mouse | 74521 | Ppp4r4 | protein phosphatase 4, regu... | XM_006516312.2 | 77.3% | 79.4% | (many diffs) |
| 17 | mouse | 74521 | Ppp4r4 | protein phosphatase 4, regu... | XR_381552.2 | 68.6% | (many diffs) | |
| 18 | mouse | 74521 | Ppp4r4 | protein phosphatase 4, regu... | XM_006516314.3 | 65.5% | 66.3% | (many diffs) |
| 19 | mouse | 74521 | Ppp4r4 | protein phosphatase 4, regu... | XM_006516315.3 | 64.4% | 66.1% | (many diffs) |
| 20 | mouse | 74521 | Ppp4r4 | protein phosphatase 4, regu... | XR_001780496.1 | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2685
- ORF length:
- 2619
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca tccgccgccg cccgccgccg cgatggattt cagtcagaac agcctgttcg 121 gttacatgga ggacctgcag gagctcacca tcatcgagag gccggtccgc cggagcctca 181 agacaccgga agaaatagaa agattgacag tcgatgaaga cctcagtgat attgaaaggg 241 ctgtttatct gctcagtgct ggtcaagatg tccaaggaac aagtgtgatt gcaaatctcc 301 catttttgat gcgacagaat cccactgaga cgcttcggag agtgttgcca aaagtcagag 361 aagccctgca tgttgcagga gtggaaatgc agttaacggc tgcgatgtca tttctgacca 421 ttctgcagga cgaatcagtg tcaattcatg catataccca ctcattcctc caagtcattc 481 tcctgcatct ggagcacagg gacacaggtg tcagcaatgc atggctggaa actcttctgt 541 ctgttataga agtattgcca aaagaaaccc tacggcatga gattttgaat ccacttgttt 601 ccaaggcaca actttcccaa acagtccagt ctcgtttagt tagttgtaaa attttaggaa 661 aattgaccaa caaatttgat gcccacacca ttaagcgaga aatacttcct ctggtaaaat 721 cactctgtca agatgtagaa tatgaagttc gatcttgtat gtgtcggcaa ttagaaaata 781 tagcccaggg cattgggaca gaacttacaa aaagtgtggt gctccctgaa ttaatagaac 841 tttctaggga tgaaggcagc agtgtacgac ttgcagcttt tgaaactttg gttaatctgc 901 ttgatatatt tgatacagat gacagaagtc aaactatact tcccttagtg aaatcatttt 961 gtgaaaaatc tttcaaagca gatgaatcaa ttcttatttc tttatctttc catttaggaa 1021 aactatgtca tggactatat ggaattttca ctccagatca gcacttgaga tttttggaat 1081 tttataagaa actttgtaca ttgggtttgc aacaagaaaa tggacacaat gaaaaccaga 1141 ttccacccca aatcctagag caggagaaga aatatatttc agtacggaag aactgtgctt 1201 ataactttcc ggccatgatt gtttttgttg atcctaaaaa cttccacatg gaactctatt 1261 ctacattctt ctgcctttgc catgaccctg aagtaccagt cagatacact attgctattt 1321 gcttttatga agtatctaag cttctgaatt ctggagtata tttaatacat aaagaactaa 1381 taacattatt acaagatgaa tcactggagg tactagatgc tcttatagat catcttccag 1441 aaatcttgga acttatgtct actggtggag aaagcagtgt tcaagaaaat aagttatctt 1501 ctctgcctga cttgattcca gcactcacag ctgctgaaca gcgagctgca gcctctttaa 1561 aatggagaac tcatgagaag ctacttcaga aatatgcctg cctgccacat gtcatatcaa 1621 gcgatcagat ttattaccgt ttcttacaaa gaatgttcac aatcatgatg acaaataatg 1681 ttttacctgt ccaaaaggcg gcttcacgaa ctctatgcat ttttctgcgt tataatcgta 1741 aacaagaaca gagacatgag gtcattcaaa aattaattga acaattgggc caaggaaaaa 1801 gttactggaa tagacttcga tttttggata cctgtgaatt tattatagag atattttcaa 1861 aatcattttt ctgtaaatat ttctttctac ctgctattga actgacacat gatccagtag 1921 caaatgtgag aatgaaactt tgctacctgt tgcccaaagt gaaatctact ctgaagattc 1981 ctgctgataa gcatctactt cagcagttag aaatgtgtgt gaggaaactc ctgtgtcaag 2041 aaaaagataa agatgttctg gctattgtaa aaagaactgt attagagttg gacagaatgg 2101 aaatgtctat ggatgctttt cagaaaaagt tttatgagaa agatttgttg gatcaagaga 2161 aagaaagaga agaactactt cttttggaaa tggaacaatt agagaaagaa aagcaacaga 2221 atgatggaag gcccatgagt gataaaatgt ttgaaaagaa acgtagagac actaagacac 2281 caacgcaaag tctgcccaag aacatcccca tttctgttcc tggaccctct tctgtcaccc 2341 catcgacaag taaagaaatc aagaaatcca aactgattcg aagccagtct tttaataatc 2401 aagcttttca tgcaaaatat ggcaacttag agaaatgtgc tagtaaaagt tctacaacag 2461 gatatacaac ttctgtctca gggttaggaa agacttctgt gctttcacta gctgatgatt 2521 cattccggac tcgtaatgcc agtagcgttc catcttcctt ttctcctaat actcccttac 2581 cgagtacttc ccgtgggaca ggtaactcag ttgaccccaa gagcagtgga agtaaagata 2641 cacaaccacg gaaggctacc ttaaaatcca gaaaatccaa tccttaccca actttcttgt 2701 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2761 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a