Construct: ORF ccsbBroad304_03907

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017468.1_s304c1, BRDN0000401743
Derived from:
ccsbBroadEn_03907
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BLOC1S5 (63915)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03907
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 63915 BLOC1S5 biogenesis of lysosomal org... NM_201280.3 100% 100%
2 human 63915 BLOC1S5 biogenesis of lysosomal org... NM_001199322.1 65.5% 65.2% 1_1delAins191;3_4insAA
3 human 63915 BLOC1S5 biogenesis of lysosomal org... NM_001199323.1 48.1% 37.1% 195_196ins130;270_271ins161
4 human 100526837 EEF1E1-BLOC1S5 EEF1E1-BLOC1S5 readthrough ... NR_037618.1 17.9% (many diffs)
5 human 100526836 BLOC1S5-TXNDC5 BLOC1S5-TXNDC5 readthrough ... NR_037616.1 14.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
630
ORF length:
561
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gagtggcgga gggacagaga cccctgtggg ttgtgaggcc gccccgggcg
121gtggcagcaa gaagagggac tccctgggga ctgcgggctc agcgcacctc attatcaagg
181atcttggaga aattcattca aggcttttgg atcacagacc agttattcaa ggtgaaactc
241gttattttgt aaaagaattt gaagaaaaac gtggtcttcg agaaatgcga gttcttgaaa
301atttgaagaa catgatccat gaaacaaatg aacatactct tcccaaatgt agagacacaa
361tgcgggacag cctcagccag gttctccaga gattgcaagc agctaatgac tcagtctgta
421gactccaaca gagggaacag gaacgaaaaa agattcatag tgaccactta gtagctagtg
481agaaacagca tatgctccag tgggacaact tcatgaagga gcaacccaac aaaagggctg
541aagtggatga agagcacaga aaagccatgg aaaggcttaa agaacaatat gctgagatgg
601agaaggacct agcgaaattt tcaacctttt tgccaacttt cttgtacaaa gtggttggta
661agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
721ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)