Construct: ORF ccsbBroad304_03909
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010662.1_s304c1, BRDN0000402688
- Derived from:
- ccsbBroadEn_03909
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS14 (63931)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03909
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63931 | MRPS14 | mitochondrial ribosomal pro... | NM_022100.3 | 100% | 100% | |
| 2 | human | 63931 | MRPS14 | mitochondrial ribosomal pro... | NR_037606.1 | 17% | 1_47del;92_183del;524_2252del | |
| 3 | mouse | 64659 | Mrps14 | mitochondrial ribosomal pro... | NM_025474.4 | 86.4% | 86.7% | (many diffs) |
| 4 | mouse | 64659 | Mrps14 | mitochondrial ribosomal pro... | NM_001347121.1 | 79.8% | 76.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccttcatg ctgggctcgc tgctgcggac gttcaagcag atggttcctt 121 catcagcttc aggccaagtt cgaagtcact atgtagactg gagaatgtgg cgcgatgtga 181 agagacgaaa aatggcctat gaatacgcag atgagaggct acgtattaat tcactcagga 241 agaataccat tttgccaaaa attcttcagg atgtggctga tgaagaaatt gctgccctcc 301 cccgggatag ctgtcctgtt agaatcagaa atcggtgtgt tatgacgtcc cgtccgcgtg 361 gtgtgaagcg gcgctggagg cttagtcgta tagtcttccg tcacttagct gaccatgggc 421 aactttctgg gatccagcga gcgacatggt acccagcttt cttgtacaaa gtggttggta 481 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 541 ggtggcgcgt taagtcgaca atcaacctct ggatta