Construct: ORF ccsbBroad304_03934

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017162.1_s304c1, BRDN0000461709
Derived from:
ccsbBroadEn_03934
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
P3H1 (64175)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03934
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64175 P3H1 prolyl 3-hydroxylase 1 NM_001146289.1 100% 100%
2 human 64175 P3H1 prolyl 3-hydroxylase 1 NM_022356.4 93% 92.8% 2048_2049insCGAGCGGGTGAGAGCAGCT;2073_2208del
3 human 64175 P3H1 prolyl 3-hydroxylase 1 NM_001243246.1 86.5% 86% (many diffs)
4 human 64175 P3H1 prolyl 3-hydroxylase 1 XR_946739.2 86.3% 1_57del;1281_1348del;2039_2040ins177
5 human 64175 P3H1 prolyl 3-hydroxylase 1 XM_005271110.2 47.7% 47.4% 0_1ins1008;1040_1041insCGAGCGGGTGAGAGCAGCT;1065_1200del
6 human 64175 P3H1 prolyl 3-hydroxylase 1 XM_017002052.2 45.7% 43.7% (many diffs)
7 human 64175 P3H1 prolyl 3-hydroxylase 1 XM_011541948.1 45.6% 43.8% (many diffs)
8 human 64175 P3H1 prolyl 3-hydroxylase 1 XM_017002051.2 45.6% 43.8% (many diffs)
9 mouse 56401 P3h1 prolyl 3-hydroxylase 1 NM_019783.2 81.5% 82.2% (many diffs)
10 mouse 56401 P3h1 prolyl 3-hydroxylase 1 NM_001286148.1 80.7% 81.3% (many diffs)
11 mouse 56401 P3h1 prolyl 3-hydroxylase 1 NM_019782.3 80.5% 81.2% (many diffs)
12 mouse 56401 P3h1 prolyl 3-hydroxylase 1 NM_001042411.1 62.2% 64.5% (many diffs)
13 mouse 56401 P3h1 prolyl 3-hydroxylase 1 XR_001784167.1 55.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2160
ORF length:
2091
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggtacgc gcgttgaagc tgctgaccac actgctggct gtcgtggccg
121ctgcctccca agccgaggtc gagtccgagg caggatgggg catggtgacg cctgatctgc
181tcttcgccga ggggaccgca gcctacgcgc gcggggactg gcccggggtg gtcctgagca
241tggaacgggc gctgcgctcc cgggcagccc tccgcgccct tcgcctgcgc tgccgcaccc
301agtgtgccgc cgacttcccg tgggagctgg accccgactg gtcccccagc ccggcccagg
361cctcgggcgc cgccgccctg cgcgacctga gcttcttcgg gggccttctg cgtcgcgctg
421cctgcctgcg ccgctgcctc gggccgccgg ccgcccactc gctcagcgaa gagatggagc
481tggagttccg caagcggagc ccctacaact acctgcaggt cgcctacttc aagatcaaca
541agttggagaa agctgttgct gcagcacaca ccttcttcgt gggcaatcct gagcacatgg
601aaatgcagca gaacctagac tattaccaaa ccatgtctgg agtgaaggag gccgacttca
661aggatcttga gactcaaccc catatgcaag aatttcgact gggagtgcga ctctactcag
721aggaacagcc acaggaagct gtgccccacc tagaggcggc gctgcaagaa tactttgtgg
781cctatgagga gtgccgtgcc ctctgcgaag ggccctatga ctacgatggc tacaactacc
841ttgagtacaa cgctgacctc ttccaggcca tcacagatca ttacatccag gtcctcaact
901gtaagcagaa ctgtgtcacg gagcttgctt cccacccaag tcgagagaag ccctttgaag
961acttcctccc atcgcattat aattatctgc agtttgccta ctataacatt gggaattata
1021cacaggctgt tgaatgtgcc aagacctatc ttctcttctt ccccaatgac gaggtgatga
1081accaaaattt ggcctattat gcagctatgc ttggagaaga acacaccaga tccatcggcc
1141cccgtgagag tgccaaggag taccgacagc gaagcctact ggaaaaagaa ctgcttttct
1201tcgcttatga tgtttttgga attccctttg tggatccgga ttcatggact ccagaagaag
1261tgattcccaa gagattgcaa gagaaacaga agtcagaacg ggaaacagcc gtacgcatct
1321cccaggagat tgggaacctt atgaaggaaa tcgagaccct tgtggaagag aagaccaagg
1381agtcactgga tgtgagcaga ctgacccggg aaggtggccc cctgctgtat gaaggcatca
1441gtctcaccat gaactccaaa ctcctgaatg gttcccagcg ggtggtgatg gacggcgtaa
1501tctctgacca cgagtgtcag gagctgcaga gactgaccaa tgtggcagca acctcaggag
1561atggctaccg gggtcagacc tccccacata ctcccaatga aaagttctat ggtgtcactg
1621tcttcaaagc cctcaagctg gggcaagaag gcaaagttcc tctgcagagt gcccacctgt
1681actacaacgt gacggagaag gtgcggcgca tcatggagtc ctacttccgc ctggatacgc
1741ccctctactt ttcctactct catctggtgt gccgcactgc catcgaagag gtccaggcag
1801agaggaagga tgatagtcat ccagtccacg tggacaactg catcctgaat gccgagaccc
1861tcgtgtgtgt caaagagccc ccagcctaca ccttccgcga ctacagcgcc atcctttacc
1921taaatgggga cttcgatggc ggaaactttt atttcactga actggatgcc aagaccgtga
1981cggcagaggt gcagcctcag tgtggaagag ccgtgggatt ctcttcaggc actgaaaacc
2041cacatggagt gaaggctgtc accagggggc agcgctgtgc catcgccctg tggttcaccc
2101tggaccctcg acacagcgag cgggtgagag cagctcgagc gggacagggt gcaggcagat
2161tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
2221attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
2281ggatta

Download FASTA (ORF) (Full)