Construct: ORF ccsbBroad304_03961
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008092.1_s304c1, BRDN0000462026
- Derived from:
- ccsbBroadEn_03961
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMURF2 (64750)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03961
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64750 | SMURF2 | SMAD specific E3 ubiquitin ... | NM_022739.4 | 100% | 100% | |
| 2 | human | 64750 | SMURF2 | SMAD specific E3 ubiquitin ... | XM_005257585.3 | 98.2% | 98.1% | 52_53ins39 |
| 3 | human | 107984974 | LOC107984974 | uncharacterized LOC107984974 | XM_024450542.1 | 18.7% | 12.8% | (many diffs) |
| 4 | human | 107984974 | LOC107984974 | uncharacterized LOC107984974 | XM_024450543.1 | 17.2% | 12.1% | (many diffs) |
| 5 | human | 107984974 | LOC107984974 | uncharacterized LOC107984974 | XM_024450545.1 | 14.8% | 11.2% | (many diffs) |
| 6 | human | 107984974 | LOC107984974 | uncharacterized LOC107984974 | XM_024450546.1 | 13.4% | 6.8% | (many diffs) |
| 7 | human | 107984974 | LOC107984974 | uncharacterized LOC107984974 | NM_001350579.1 | 11.2% | 3.4% | (many diffs) |
| 8 | human | 107984974 | LOC107984974 | uncharacterized LOC107984974 | NM_001350578.1 | 10.7% | 3.8% | (many diffs) |
| 9 | human | 107984974 | LOC107984974 | uncharacterized LOC107984974 | XM_024450547.1 | 10% | 3.4% | (many diffs) |
| 10 | mouse | 66313 | Smurf2 | SMAD specific E3 ubiquitin ... | NM_025481.2 | 92% | 99.1% | (many diffs) |
| 11 | mouse | 66313 | Smurf2 | SMAD specific E3 ubiquitin ... | XM_006533936.3 | 90.3% | 97.3% | (many diffs) |
| 12 | mouse | 66313 | Smurf2 | SMAD specific E3 ubiquitin ... | XM_017314705.1 | 62% | 67.2% | (many diffs) |
| 13 | mouse | 66313 | Smurf2 | SMAD specific E3 ubiquitin ... | XR_001780039.1 | 39.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2313
- ORF length:
- 2244
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaagg 61 ttggcaccat gtctaacccc ggaggccgga ggaacgggcc cgtcaagctg cgcctgacag 121 tactctgtgc aaaaaacctg gtgaaaaagg attttttccg acttcctgat ccatttgcta 181 aggtggtggt tgatggatct gggcaatgcc attctacaga tactgtgaag aatacgcttg 241 atccaaagtg gaatcagcat tatgacctgt atattggaaa gtctgattca gttacgatca 301 gtgtatggaa tcacaagaag atccataaga aacaaggtgc tggatttctc ggttgtgttc 361 gtcttctttc caatgccatc aaccgcctca aagacactgg ttatcagagg ttggatttat 421 gcaaactcgg gccaaatgac aatgatacag ttagaggaca gatagtagta agtcttcagt 481 ccagagaccg aataggcaca ggaggacaag ttgtggactg cagtcgttta tttgataacg 541 atttaccaga cggctgggaa gaaaggagaa ccgcctctgg aagaatccag tatctaaacc 601 atataacaag aactacgcaa tgggagcgcc caacacgacc ggcatccgaa tattctagcc 661 ctggcagacc tcttagctgc tttgttgatg agaacactcc aattagtgga acaaatggtg 721 caacatgtgg acagtcttca gatcccaggc tggcagagag gagagtcagg tcacaacgac 781 atagaaatta catgagcaga acacatttac atactcctcc agacctacca gaaggctatg 841 aacagaggac aacgcaacaa ggccaggtgt atttcttaca tacacagact ggtgtgagca 901 catggcatga tccaagagtg cccagggatc ttagcaacat caattgtgaa gagcttggtc 961 cattgcctcc tggatgggag atccgtaata cggcaacagg cagagtttat ttcgttgacc 1021 ataacaacag aacaacacaa tttacagatc ctcggctgtc tgctaacttg catttagttt 1081 taaatcggca gaaccaattg aaagaccaac agcaacagca agtggtatcg ttatgtcctg 1141 atgacacaga atgcctgaca gtcccaaggt acaagcgaga cctggttcag aaactaaaaa 1201 ttttgcggca agaactttcc caacaacagc ctcaggcagg tcattgccgc attgaggttt 1261 ccagggaaga gatttttgag gaatcatatc gacaggtcat gaaaatgaga ccaaaagatc 1321 tctggaagcg attaatgata aaatttcgtg gagaagaagg ccttgactat ggaggcgttg 1381 ccagggaatg gttgtatctc ttgtcacatg aaatgttgaa tccatactat ggcctcttcc 1441 agtattcaag agatgatatt tatacattgc agatcaatcc tgattctgca gttaatccgg 1501 aacatttatc ctatttccac tttgttggac gaataatggg aatggctgtg tttcatggac 1561 attatattga tggtggtttc acattgcctt tttataagca attgcttggg aagtcaatta 1621 ccttggatga catggagtta gtagatccgg atcttcacaa cagtttagtg tggatacttg 1681 agaatgatat tacaggtgtt ttggaccata ccTTCTGTGT TGAACATAAT GCATATGGTG 1741 AAATTATTCA GCATGAACTT AAACCAAATG GCAAAAGTAT CCCTGTTAAT GAAGAAAATA 1801 AAAAAGAATA TGTCAGGCTC TATGTGAACT GGAGATTTTT ACGAGGCATT GAGGCTCAAT 1861 TCTTGGCTCT GCAGAAAGGA TTTAATGAAG TAATTCCACA ACATCTGCTG AAGACATTTG 1921 ATGAGAAGGA GTTAGAGCTC ATTATTTGTG GACTTGGAAA GATAGATGTT AATGACTGGA 1981 AGGTAAACAC CCGGTTAAAA CACTGTACAC CAGACAGCAA CATTGTCAAA TGGTTCTGGA 2041 AAGCTGTGGA GTTTTTTGAT GAAGAGCGAC GAGCAAGATT GCTTCAGTTT GTGACAGGAT 2101 CCTCTCGAGT GCCTCTGCAG GGCTTCAAAG CATTGCAAGG TGCTGCAGGC CCGAGACTCT 2161 TTACCATACA CCAGATTGAT GCCTGCACTA ACAACCTGCC GAAAGCCCAC ACTTGCTTCA 2221 ATCGAATAGA CATTCCACCC TATGAAAGCT ATGAAAAGCT ATATGAAAAG CTGCTAACAG 2281 CCATTGAAGA AACATGTGGA TTTGCTGTGG AATTGCCAAC TTTCTTGTAC aaagtggttg 2341 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2401 accggtggcg cgttaagtcg acaatcaacc tctggatta