Construct: ORF ccsbBroad304_03982
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014631.1_s304c1, BRDN0000401534
- Derived from:
- ccsbBroadEn_03982
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAPOLG (64895)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03982
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64895 | PAPOLG | poly(A) polymerase gamma | NM_022894.4 | 100% | 100% | |
| 2 | human | 64895 | PAPOLG | poly(A) polymerase gamma | XM_005264500.4 | 97% | 97% | 1988_1989ins66 |
| 3 | human | 64895 | PAPOLG | poly(A) polymerase gamma | XM_005264501.2 | 94% | 94% | 0_1ins132 |
| 4 | human | 64895 | PAPOLG | poly(A) polymerase gamma | XM_011533041.1 | 67.6% | 67.6% | 0_1ins714 |
| 5 | human | 64895 | PAPOLG | poly(A) polymerase gamma | XM_017004723.2 | 67.6% | 67.6% | 0_1ins714 |
| 6 | human | 64895 | PAPOLG | poly(A) polymerase gamma | XM_017004724.2 | 64.6% | 64.6% | 0_1ins714;1274_1275ins66 |
| 7 | mouse | 216578 | Papolg | poly(A) polymerase gamma | NM_172555.2 | 91% | 93.6% | (many diffs) |
| 8 | mouse | 216578 | Papolg | poly(A) polymerase gamma | XM_006514653.3 | 89.9% | 92.5% | (many diffs) |
| 9 | mouse | 216578 | Papolg | poly(A) polymerase gamma | XR_380964.3 | 33.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2277
- ORF length:
- 2208
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaagagatg tctgcaaaca ccgtgctgga cagccagcgt caacaaaagc 121 attatggaat tacctcccca attagtttgg catctcctaa agaaattgat catatttaca 181 cacagaaatt aattgacgcc atgaaaccat ttggagtgtt tgaagatgag gaagaattga 241 accacaggct ggtggttctt ggtaaattga acaatttagt aaaagaatgg atttctgatg 301 tcagcgagag taagaacctc ccaccttctg ttgtggctac tgttggtggt aaaattttca 361 catttggatc ctataggctt ggagtacaca ccaaaggagc tgacattgat gcactttgtg 421 tagctccaag acatgtggaa agatctgatt tttttcagtc tttttttgaa aaattgaaac 481 atcaagatgg cattagaaac ttaagagctg tagaagatgc ctttgtacct gttataaaat 541 ttgaatttga tggtattgaa attgatctag tctttgcaag actggcaata caaaccatat 601 cagataattt agatctaaga gacgactctc gcctgagaag ccttgatata aggtgtattc 661 gcagcttaaa tggttgtaga gttactgatg aaattttgca tttagtgcca aataaagaaa 721 cttttagact caccctaaga gctgtcaaat tatgggcaaa acgacgtggt atttattcca 781 acatgctagg attccttggt ggtgtctcct gggcaatgct agttgcaaga acttgccaat 841 tgtatccaaa tgcagcagca tctactttag ttcataagtt ctttttagtt ttttccaagt 901 gggaatggcc aaatcctgtg ctgctgaagc aaccagaaga aagcaatttg aatttgcctg 961 tctgggatcc tcgggtaaat ccatcagata ggtatcatct catgcccata atcacccctg 1021 cctacccaca acagaattct acgtataatg tgtccacatc aactcgaaca gtaatggtag 1081 aagaatttaa acaaggtctt gcagtcacag atgaaattct tcaaggaaag tcagattggt 1141 ccaaactact tgagccaccg aatttctttc aaaagtatag acattatata gtattgactg 1201 ccagcgcatc aacagaagaa aaccatctag agtgggttgg attagtagaa tctaaaatcc 1261 gtgtacttgt tggaaacttg gaacggaatg aatttattac tcttgcccat gtgaatcccc 1321 agtcattccc agggaataag gaacatcata aagacaacaa ttacgtatca atgtggttcc 1381 ttgggataat ttttcggaga gtagaaaatg cagaaagtgt caacatagac ttgacatatg 1441 atatacagtc atttactgat acagtgtaca gacaggcaaa caatataaat atgctaaagg 1501 agggaatgaa aattgaagca actcatgtaa agaaaaaaca acttcaccac taccttcctg 1561 cagaaattct tcaaaagaag aaaaagcaaa gtctctctga tgtcaatcga agctcgggcg 1621 gacttcaatc caaaagattg tctctggata gcagttgtct ggatagctcc agagacactg 1681 ataatggaac accttttaat tctccagcgt ccaagtctga tagcccttct gtaggagaaa 1741 cagaaaggaa tagtgctgag cctgctgctg taattgtgga gaagccactg agtgtaccac 1801 cagcccaagg actttccatt ccagtgattg gcgcaaaagt tgactctaca gtaaaaactg 1861 tatcaccccc cactgtgtgt accattccta ccgtagtagg acgaaatgtc attcctagaa 1921 tcacaacacc tcacaaccct gcccagggac aaccgcatct gaatggaatg tcaaatataa 1981 ctaagactgt tacacctaag agatcccatt ccccatccat agatgggact cctaagaggt 2041 tgaaagacgt agaaaagttt attcgacttg aatcaacatt taaggacccc cgcactgctg 2101 aagaaagaaa aagaaaatca gtggatgcca ttggaggaga atctatgcct attccaacta 2161 ttgatacatc acgcaaaaag agactaccca gtaaagaact accagattca tcatctccag 2221 ttccagcaaa caacatccgt gtcatcaaaa attccattcg actgaccctt aatcggttgc 2281 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2341 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2401 tta