Construct: ORF ccsbBroad304_03985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006124.1_s304c1, BRDN0000410838
- Derived from:
- ccsbBroadEn_03985
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL14 (64928)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03985
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64928 | MRPL14 | mitochondrial ribosomal pro... | NM_001318767.1 | 100% | 100% | |
| 2 | human | 64928 | MRPL14 | mitochondrial ribosomal pro... | NM_001318768.1 | 100% | 100% | |
| 3 | human | 64928 | MRPL14 | mitochondrial ribosomal pro... | NM_032111.4 | 100% | 100% | |
| 4 | human | 64928 | MRPL14 | mitochondrial ribosomal pro... | NM_001318769.1 | 92.9% | 92.9% | 1_33del |
| 5 | human | 64928 | MRPL14 | mitochondrial ribosomal pro... | NM_001318771.1 | 76.5% | 76.5% | 0_1ins102 |
| 6 | human | 64928 | MRPL14 | mitochondrial ribosomal pro... | NM_001318770.1 | 73.2% | 73.2% | 1_159del |
| 7 | mouse | 68463 | Mrpl14 | mitochondrial ribosomal pro... | XM_017317618.1 | 66.2% | 68.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tttctttact gggctctggg gccccttcac ctgtgtaagc agagtgctga 121 gccatcactg tttcagcacc actgggagtc tgagtgcgat tcagaagatg acgcgggtac 181 gagtggtgga caacagtgcc ctggggaaca gcccatacca tcgggctcct cgctgcatcc 241 atgtctataa gaagaatgga gtgggcaagg tgggcgacca gatactactg gccatcaagg 301 gacagaagaa aaaggcgctc attgtggggc actgcatgcc tggcccccga atgaccccca 361 gattcgactc caacaacgtg gtcctcattg aggacaacgg gaaccctgtg gggacacgaa 421 ttaagacacc catccccacc agcctgcgca agcgggaagg cgagtattcc aaggtgctgg 481 ccattgctca gaactttgtg tgcccaactt tcttgtacaa agtggttggt aagcctatcc 541 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 601 ttaagtcgac aatcaacctc tggatta