Construct: ORF ccsbBroad304_03998
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016218.1_s304c1, BRDN0000461541
- Derived from:
- ccsbBroadEn_03998
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPATS2 (65244)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03998
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65244 | SPATS2 | spermatogenesis associated ... | NM_001293285.1 | 100% | 100% | |
| 2 | human | 65244 | SPATS2 | spermatogenesis associated ... | NM_001293286.1 | 100% | 100% | |
| 3 | human | 65244 | SPATS2 | spermatogenesis associated ... | NM_023071.3 | 100% | 100% | |
| 4 | human | 65244 | SPATS2 | spermatogenesis associated ... | XM_011538691.3 | 100% | 100% | |
| 5 | human | 65244 | SPATS2 | spermatogenesis associated ... | XM_017019840.2 | 83.4% | 81% | 1324_1325ins134;1365_1366ins136 |
| 6 | human | 65244 | SPATS2 | spermatogenesis associated ... | XM_024449135.1 | 83.4% | 81% | 1324_1325ins134;1365_1366ins136 |
| 7 | human | 65244 | SPATS2 | spermatogenesis associated ... | NR_120663.1 | 42.5% | 1_989del;1828_1871del;2669_3847del | |
| 8 | human | 65244 | SPATS2 | spermatogenesis associated ... | XR_002957371.1 | 33.7% | 1_326del;1165_2857del;3655_4843del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1701
- ORF length:
- 1635
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc caggaaacag aaccagaagg attcatcagg attcattttt gatttgcagt 121 ccaataccgt actggcccag ggaggagctt ttgagaacat gaaagagaag ataaatgcgg 181 tacgtgcaat agttcctaat aagagcaaca atgaaattat cctggttttg cagcactttg 241 ataactgtgt ggacaaaaca gtacaagcat tcatggaagg tagtgccagt gaagtactca 301 aagaatggac agtaacaggc aagaaaaaga acaaaaagaa gaaaaacaaa ccgaaacctg 361 ccgcagaacc aagtaacggc atcccagatt ccagtaaatc agtttccatt caagaggaac 421 agtctgcgcc ttcctcagag aaaggtggta tgaatggcta ccatgtcaat ggtgccatca 481 atgacactga gtctgtggac tcactcagtg aaggtttgga gacactttca atagatgcca 541 gagaattgga ggatcccgag tctgccatgc tagatacgct ggatagaaca ggatccatgc 601 tgcagaatgg tgtctctgat tttgagacca agtctttgac tatgcactct attcacaatt 661 ctcaacaacc caggaatgct gccaaatctc tctcaagacc taccacagaa actcagtttt 721 caaatatggg gatggaagat gttcccctcg ccaccagtaa aaagctaagt tccaatattg 781 aaaaatctgt aaaagacctc cagcgctgca cagtgtctct tgcacggtat cgagttgtag 841 ttaaagaaga gatggatgcc tccattaaga aaatgaaaca agcctttgct gaattggaga 901 gctgtttaat ggatcgagaa gtggcgttgc ttgctgaaat ggacaaagtg aaagctgaag 961 caatggaaat tttgctcagc cgacaaaaga aggctgaact tctaaagaag atgactcatg 1021 tggctgttca aatgtcagag cagcaattgg ttgagctcag agctgatatc aagcactttg 1081 ttagtgaacg taaatatgat gaggatctgg gacgagtagc ccggttcacc tgtgatgtag 1141 agaccctaaa gaagagcatt gattcatttg gacaagtgtc tcatccaaag aacagctatt 1201 cgaccagatc ccgatgtagc tcagttacat ctgtgtcctt gagtagccca agtgatgcct 1261 ctgctgcttc ctcttccacc tgtgcctctc ctcccagcct tacaagtgct aacaagaaaa 1321 actttgcacc gggagagact cctgcagcca tagcaaactc cagtggccag ccctaccagc 1381 cacttcggga ggtattgcca gggaacagac gaggaggaca gggctatagg ccacaaggcc 1441 aaaagtccaa tgaccccatg aaccaagggc ggcatgacag tatgggtcgt tacagaaaca 1501 gctcgtggta ttcatctggt tccaggtatc agagtgctcc atctcaggca ccaggaaaca 1561 ccattgaaag aggccagact cactctgcag ggaccaatgg aactggagtc agcatggagc 1621 ccagccctcc cacgccttca ttcaaaaagg ggctccccca gcgcaaaccc aggacctctc 1681 agactgaagc cgtgaactct tgcccaactt tcttgtacaa agtggttggt aagcctatcc 1741 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1801 ttaagtcgac aatcaacctc tggatta