Transcript: Human XM_017019840.2

PREDICTED: Homo sapiens spermatogenesis associated serine rich 2 (SPATS2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SPATS2 (65244)
Length:
2039
CDS:
430..1797

Additional Resources:

NCBI RefSeq record:
XM_017019840.2
NBCI Gene record:
SPATS2 (65244)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017019840.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000140711 GCACGGTATCGAGTTGTAGTT pLKO.1 1186 CDS 100% 4.950 6.930 N SPATS2 n/a
2 TRCN0000281055 GCACGGTATCGAGTTGTAGTT pLKO_005 1186 CDS 100% 4.950 6.930 N SPATS2 n/a
3 TRCN0000144331 CCTTACAAGTGCTAACAAGAA pLKO.1 1662 CDS 100% 4.950 3.465 N SPATS2 n/a
4 TRCN0000280987 CCTTACAAGTGCTAACAAGAA pLKO_005 1662 CDS 100% 4.950 3.465 N SPATS2 n/a
5 TRCN0000145180 GCACTTTGTTAGTGAACGTAA pLKO.1 1437 CDS 100% 4.950 3.465 N SPATS2 n/a
6 TRCN0000281054 GCACTTTGTTAGTGAACGTAA pLKO_005 1437 CDS 100% 4.950 3.465 N SPATS2 n/a
7 TRCN0000139273 CCCGATGTAGCTCAGTTACAT pLKO.1 1574 CDS 100% 0.563 0.394 N SPATS2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017019840.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03998 pDONR223 100% 83.4% 81% None 1324_1325ins134;1365_1366ins136 n/a
2 ccsbBroad304_03998 pLX_304 0% 83.4% 81% V5 1324_1325ins134;1365_1366ins136 n/a
3 TRCN0000477020 AATAGTCAGCGCGCCTAGGACAAC pLX_317 20.4% 83.4% 81% V5 1324_1325ins134;1365_1366ins136 n/a
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