Transcript: Human XR_002957371.1

PREDICTED: Homo sapiens spermatogenesis associated serine rich 2 (SPATS2), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SPATS2 (65244)
Length:
4843
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002957371.1
NBCI Gene record:
SPATS2 (65244)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002957371.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000140711 GCACGGTATCGAGTTGTAGTT pLKO.1 1083 3UTR 100% 4.950 6.930 N SPATS2 n/a
2 TRCN0000281055 GCACGGTATCGAGTTGTAGTT pLKO_005 1083 3UTR 100% 4.950 6.930 N SPATS2 n/a
3 TRCN0000143881 CCCAATGCCTTGTTTCCTATT pLKO.1 4257 3UTR 100% 10.800 7.560 N SPATS2 n/a
4 TRCN0000144331 CCTTACAAGTGCTAACAAGAA pLKO.1 3252 3UTR 100% 4.950 3.465 N SPATS2 n/a
5 TRCN0000280987 CCTTACAAGTGCTAACAAGAA pLKO_005 3252 3UTR 100% 4.950 3.465 N SPATS2 n/a
6 TRCN0000145180 GCACTTTGTTAGTGAACGTAA pLKO.1 3027 3UTR 100% 4.950 3.465 N SPATS2 n/a
7 TRCN0000281054 GCACTTTGTTAGTGAACGTAA pLKO_005 3027 3UTR 100% 4.950 3.465 N SPATS2 n/a
8 TRCN0000144984 GCCTTGTTTCCTATTACCTTA pLKO.1 4263 3UTR 100% 4.950 3.465 N SPATS2 n/a
9 TRCN0000281053 GCCTTGTTTCCTATTACCTTA pLKO_005 4263 3UTR 100% 4.950 3.465 N SPATS2 n/a
10 TRCN0000139273 CCCGATGTAGCTCAGTTACAT pLKO.1 3164 3UTR 100% 0.563 0.394 N SPATS2 n/a
11 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2198 3UTR 100% 4.950 2.475 Y CFLAR n/a
12 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2198 3UTR 100% 4.950 2.475 Y C19orf31 n/a
13 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4369 3UTR 100% 13.200 6.600 Y LIAS n/a
14 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 4441 3UTR 100% 4.950 2.475 Y LOC339059 n/a
15 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2196 3UTR 100% 4.950 2.475 Y ERN2 n/a
16 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2196 3UTR 100% 4.950 2.475 Y P3H4 n/a
17 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2196 3UTR 100% 4.950 2.475 Y P3H4 n/a
18 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 4533 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002957371.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03998 pDONR223 100% 33.7% None 1_326del;1165_2857del;3655_4843del n/a
2 ccsbBroad304_03998 pLX_304 0% 33.7% V5 1_326del;1165_2857del;3655_4843del n/a
3 TRCN0000477020 AATAGTCAGCGCGCCTAGGACAAC pLX_317 20.4% 33.7% V5 1_326del;1165_2857del;3655_4843del n/a
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