Construct: ORF ccsbBroad304_04100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016158.1_s304c1, BRDN0000409821
- Derived from:
- ccsbBroadEn_04100
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM204 (79652)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04100
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79652 | TMEM204 | transmembrane protein 204 | NM_001256541.2 | 100% | 100% | |
| 2 | human | 79652 | TMEM204 | transmembrane protein 204 | NM_024600.6 | 100% | 100% | |
| 3 | mouse | 407831 | Tmem204 | transmembrane protein 204 | NM_001001183.1 | 83.5% | 88% | (many diffs) |
| 4 | mouse | 407831 | Tmem204 | transmembrane protein 204 | XM_006524578.1 | 83.5% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac cgtgcagaga ctcgtggccg cggccgtgct ggtggccctg gtctcactca 121 tcctcaacaa cgtggcggcc ttcacctcca actgggtgtg ccagacgctg gaggatgggc 181 gcaggcgcag cgtggggctg tggaggtcct gctggctggt ggacaggacc cggggagggc 241 cgagccctgg ggccagagcc ggccaggtgg acgcacatga ctgtgaggcg ctgggctggg 301 gctccgaggc agccggcttc caggagtccc gaggcaccgt caaactgcag ttcgacatga 361 tgcgcgcctg caacctggtg gccacggccg cgctcaccgc aggccagctc accttcctcc 421 tggggctggt gggcctgccc ctgctgtcac ccgacgcccc gtgctgggag gaggccatgg 481 ccgctgcatt ccaactggcg agttttgtcc tggtcatcgg gctcgtgact ttctacagaa 541 ttggcccata caccaacctg tcctggtcct gctacctgaa cattggcgcc tgccttctgg 601 ccacgctggc ggcagccatg ctcatctgga acattctcca caagagggag gactgcatgg 661 ccccccgggt gattgtcatc agccgctccc tgacagcgcg ctttcgccgt gggctggaca 721 atgactacgt ggagtcacca tgctgcccaa ctttcttgta caaagtggtt ggtaagccta 781 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 841 gcgttaagtc gacaatcaac ctctggatta