Construct: ORF ccsbBroad304_04120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007889.1_s304c1, BRDN0000409669
- Derived from:
- ccsbBroadEn_04120
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GEMIN7 (79760)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04120
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_001007269.2 | 100% | 100% | |
| 2 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_001007270.2 | 100% | 100% | |
| 3 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_001319054.1 | 100% | 100% | |
| 4 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_001319055.2 | 100% | 100% | |
| 5 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_024707.3 | 100% | 100% | |
| 6 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | XM_005259262.3 | 100% | 100% | |
| 7 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | XM_005259263.5 | 100% | 100% | |
| 8 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | XM_017027312.1 | 100% | 100% | |
| 9 | mouse | 69731 | Gemin7 | gem nuclear organelle assoc... | NM_027189.2 | 78.1% | 80.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca aactccagtg aacattcccg tgcctgtgct ccggctgccc cggggccctg 121 atggcttcag ccgtggcttt gcccctgatg gacgcagagc ccccttgagg ccagaggttc 181 ctgaaatcca ggagtgtccc atagctcaag aatccctgga atcccaggag cagcgggcac 241 gagccgccct tcgggagcgt tacctccgca gcctgctggc catggtgggt catcaggtga 301 gcttcacgtt gcacgagggt gtgcgtgtgg ccgcccactt tggagccacc gacctggatg 361 tggccaactt ctacgtgtca cagctgcaga ctcccatagg tgtgcaagca gaggcgctgc 421 tccgatgtag tgacattatt tcatatacct tcaagccata cccaactttc ttgtacaaag 481 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 541 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta