Construct: ORF ccsbBroad304_04154
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011963.1_s304c1, BRDN0000410316
- Derived from:
- ccsbBroadEn_04154
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DENND2D (79961)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04154
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79961 | DENND2D | DENN domain containing 2D | NM_024901.5 | 100% | 100% | |
| 2 | human | 79961 | DENND2D | DENN domain containing 2D | NM_001271833.1 | 97.2% | 96.8% | (many diffs) |
| 3 | human | 79961 | DENND2D | DENN domain containing 2D | XM_011542187.1 | 95.5% | 95.3% | (many diffs) |
| 4 | human | 79961 | DENND2D | DENN domain containing 2D | XM_011542190.2 | 95.5% | 95.3% | (many diffs) |
| 5 | human | 79961 | DENND2D | DENN domain containing 2D | XM_006710921.2 | 94.4% | 93.8% | (many diffs) |
| 6 | human | 79961 | DENND2D | DENN domain containing 2D | XM_006710922.1 | 79.7% | 67.9% | (many diffs) |
| 7 | human | 79961 | DENND2D | DENN domain containing 2D | XM_006710923.1 | 79.7% | 67.9% | (many diffs) |
| 8 | human | 79961 | DENND2D | DENN domain containing 2D | XM_006710924.1 | 79.7% | 67.9% | (many diffs) |
| 9 | human | 79961 | DENND2D | DENN domain containing 2D | XM_011542189.1 | 79.7% | 67.9% | (many diffs) |
| 10 | human | 79961 | DENND2D | DENN domain containing 2D | XM_024449890.1 | 79.7% | 67.9% | (many diffs) |
| 11 | human | 79961 | DENND2D | DENN domain containing 2D | XM_024449891.1 | 79.7% | 67.9% | (many diffs) |
| 12 | human | 79961 | DENND2D | DENN domain containing 2D | XR_946764.1 | 66.1% | (many diffs) | |
| 13 | human | 79961 | DENND2D | DENN domain containing 2D | XM_017002386.1 | 61.7% | 61.7% | 0_1ins540 |
| 14 | human | 79961 | DENND2D | DENN domain containing 2D | XM_017002387.1 | 61.7% | 61.7% | 0_1ins540 |
| 15 | human | 79961 | DENND2D | DENN domain containing 2D | XM_017002388.1 | 61.7% | 61.7% | 0_1ins540 |
| 16 | human | 79961 | DENND2D | DENN domain containing 2D | XM_024449898.1 | 61.7% | 61.7% | 0_1ins540 |
| 17 | human | 79961 | DENND2D | DENN domain containing 2D | XM_017002389.1 | 42.6% | 41.4% | (many diffs) |
| 18 | human | 79961 | DENND2D | DENN domain containing 2D | XR_946765.1 | 40.3% | (many diffs) | |
| 19 | mouse | 72121 | Dennd2d | DENN/MADD domain containing 2D | NM_001093754.2 | 85.4% | 89.3% | (many diffs) |
| 20 | mouse | 72121 | Dennd2d | DENN/MADD domain containing 2D | NM_028110.2 | 85.3% | 89.5% | (many diffs) |
| 21 | mouse | 72121 | Dennd2d | DENN/MADD domain containing 2D | XM_006502133.3 | 73.3% | 69.7% | (many diffs) |
| 22 | mouse | 72121 | Dennd2d | DENN/MADD domain containing 2D | XR_867235.2 | 58.6% | (many diffs) | |
| 23 | mouse | 72121 | Dennd2d | DENN/MADD domain containing 2D | NM_001278941.1 | 54.2% | 57.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1479
- ORF length:
- 1413
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga aggacaagtg gtaggccggg tgttcaggct cttccaacgc cgactgcttc 121 aactccgagc aggaccaccc caggacaatt caggggaagc tttaaaggaa ccagaaaggg 181 cccaggagca ctctttgccc aactttgctg gggggcagca cttctttgaa taccttcttg 241 tggtttctct caaaaagaag cgttcagagg atgattacga gcctataatc acctaccaat 301 ttcccaagcg ggagaacctg cttcggggtc agcaggagga ggaggagcgg ctgctcaaag 361 ctatcccctt gttctgcttc ccagatggga atgagtgggc atcactcacc gagtatccca 421 gggagacctt ctccttcgtt ctgaccaatg tggatgggag cagaaagatt ggatactgca 481 ggcgcctctt gcctgccggc cctggccctc gccttcccaa agtgtactgc atcatcagct 541 gcatcggctg cttcggcttg ttctccaaga tcctggatga agtggagaag agacatcaga 601 tctccatggc tgtcatctac ccgttcatgc agggcctccg agaggcagcc ttccctgctc 661 ctgggaagac tgtcactctc aagagcttca tccccgactc aggcactgag ttcatttcac 721 tgacacggcc cctggactcc cacctagaac atgtggattt tagttctcta ttgcactgtc 781 tcagttttga acagatactt cagatctttg cctctgccgt gctggagaga aaaatcatct 841 tcctggcgga aggtctcagc accttgtctc agtgcatcca tgctgctgcc gcactgctct 901 accccttcag ctgggcgcac acctacatcc ctgttgtccc tgagagcctt ctggccaccg 961 tctgctgccc cacccccttc atggttggag tacaaatgcg cttccagcag gaggtcatgg 1021 acagccctat ggaagaggtc ctgctggtca atctttgtga aggaaccttc ttaatgtcgg 1081 ttggtgatga aaaagacatc ctgccaccga agcttcagga tgacatctta gactctcttg 1141 gtcaggggat caatgagtta aagactgcag aacaaatcaa cgagcatgtt tcaggcccct 1201 ttgtgcagtt ctttgtcaag attgtgggcc attatgcttc ctatatcaag cgggaggcaa 1261 atgggcaagg ccacttccaa gaaagatcct tctgtaaggc tctgacctcc aagaccaacc 1321 gccgatttgt gaagaagttt gtgaagacac agctcttctc acttttcatc caggaagccg 1381 agaagagcaa gaatcctcct gcaggctatt tccaacagaa aatacttgaa tatgaggaac 1441 agaagaaaca gaagaaacca agggaaaaaa ctgtgaaata cccaactttc ttgtacaaag 1501 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1561 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta