Construct: ORF ccsbBroad304_04196
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002426.1_s304c1, BRDN0000400267
- Derived from:
- ccsbBroadEn_04196
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM214B (80256)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04196
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80256 | FAM214B | family with sequence simila... | NM_001317991.2 | 99.9% | 100% | 414G>A |
| 2 | human | 80256 | FAM214B | family with sequence simila... | NM_025182.4 | 99.9% | 100% | 414G>A |
| 3 | human | 80256 | FAM214B | family with sequence simila... | XM_005251588.1 | 99.9% | 100% | 414G>A |
| 4 | human | 80256 | FAM214B | family with sequence simila... | XM_005251590.1 | 99.9% | 100% | 414G>A |
| 5 | human | 80256 | FAM214B | family with sequence simila... | XM_005251591.1 | 99.9% | 100% | 414G>A |
| 6 | human | 80256 | FAM214B | family with sequence simila... | XM_011518037.1 | 99.9% | 100% | 414G>A |
| 7 | human | 80256 | FAM214B | family with sequence simila... | XM_024447689.1 | 99.9% | 100% | 414G>A |
| 8 | human | 80256 | FAM214B | family with sequence simila... | NR_134455.2 | 43.9% | (many diffs) | |
| 9 | mouse | 230088 | Fam214b | family with sequence simila... | NM_001253353.1 | 92.8% | 91% | (many diffs) |
| 10 | mouse | 230088 | Fam214b | family with sequence simila... | NM_001253354.1 | 92.8% | 91% | (many diffs) |
| 11 | mouse | 230088 | Fam214b | family with sequence simila... | NM_172691.3 | 92.8% | 91% | (many diffs) |
| 12 | mouse | 230088 | Fam214b | family with sequence simila... | XM_006537822.2 | 92.8% | 91% | (many diffs) |
| 13 | mouse | 230088 | Fam214b | family with sequence simila... | XM_006537823.3 | 92.8% | 91% | (many diffs) |
| 14 | mouse | 230088 | Fam214b | family with sequence simila... | XM_006537824.1 | 92.8% | 91% | (many diffs) |
| 15 | mouse | 230088 | Fam214b | family with sequence simila... | XM_011249994.1 | 92.8% | 91% | (many diffs) |
| 16 | mouse | 230088 | Fam214b | family with sequence simila... | XM_017320125.1 | 92.8% | 91% | (many diffs) |
| 17 | mouse | 230088 | Fam214b | family with sequence simila... | NR_045567.1 | 46.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1680
- ORF length:
- 1614
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg ccacgtgcag gcggagccgt ctccatcctc agagccggag gctggccctt 121 cacagcctcc agtcaggcag ggggccctcc agggtggcct gctcatgggc tacagcccag 181 cagggggggc gacatccccc ggggtctacc aggtatccat cttttcccct ccggctggta 241 cctctgagcc tcatagggcc ctgaaacgac aagccccatc cactgagggt ccccgggagc 301 tgaagagagg ccctgggctg ggggccagag agggactacc ccctgaagaa ccatctactg 361 tggggctctt aggcccagag ggaccggggc tgggactagg tgtggccagc cagcatttct 421 cccaccgtgg cctctgtgtt gtggaacaga gaagtagtgt cacctcatct tggacttcag 481 gggcctggag tcccccctgc cccccatcaa atgcttcctg caatactttg cacaccagag 541 actgggcctc cccagatcca gggggacagg ggtccctggg ggagtcccca gggccagccc 601 ctccaggcca gctgcacaca cttgacactg atttgcacag tcttgcacaa atagggggta 661 agagcccagt ggctggggtg ggcaatgggg gtagcctctg gcctagggag tcccctggca 721 ctgccaatgg gcacagtccc gagcacacac cccctggccc tggaccccca ggcccctgcc 781 ccaccaagcg aaggctgctt cctgctggag aagccccaga tgtcagctct gaggaagagg 841 ggccagcccc tcggaggcgc cggggatccc tgggccaccc tactgctgcc aacagttctg 901 atgccaaagc cacacccttc tggagccacc tgctgcctgg gcccaaagag cctgttttgg 961 acccaacaga ctgcggtccc atggggcgga ggctgaaagg agcccgtcgc ctgaagctga 1021 gcccccttcg aagcctccgg aaggggccag gcctgctgag cccccccagt gcctcccctg 1081 ttcctacccc tgctgtcagc cgtaccctgc tgggcaactt tgaggaatca ttgctgcgag 1141 gacgttttgc accatctggc cacattgagg gcttcacagc agaaattgga gctagtgggt 1201 catactgccc ccagcacgtc acgctgcctg tcactgtcac attctttgat gtttctgagc 1261 aaaatgcccc ggctcccttc ctgggcatcg tggatctgaa ccccttgggg aggaagggtt 1321 acagcgtgcc caaggtgggc accgtccaag tgaccttatt taaccccaac cagactgtgg 1381 taaagatgtt ccttgtgacc tttgacttct cggacatgcc tgctgcccac atgaccttcc 1441 tgcgccatcg cctctttttg gtgcctgtgg gtgaggaggg aaatgctaac cccacccacc 1501 gcctcctctg ctacttgctg cacctcaggt tccggagctc ccgctcaggc cgcttaagcc 1561 tgcatggaga tatccgcctg cttttttccc gccggagcct ggagctggac acagggctcc 1621 cctacgaact gcaggctgtg accgaggccc ctcataatcc acgttattca cctttgccct 1681 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1741 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1801 ggatta