Construct: ORF ccsbBroad304_04202
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002198.1_s304c1, BRDN0000409127
- Derived from:
- ccsbBroadEn_04202
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MED28 (80306)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04202
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80306 | MED28 | mediator complex subunit 28 | NM_025205.5 | 100% | 100% | |
| 2 | mouse | 66999 | Med28 | mediator complex subunit 28 | NM_025895.4 | 88% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctccacta gggggtatgt tttctgggca gccacccggt ccccctcagg 121 ccccgccggg ccttccgggc caagcttcgc ttcttcaggc agctccaggc gctcctagac 181 cttccagcag tactttggtg gacgagttgg agtcatcttt cgaggcttgc tttgcatctc 241 tggtgagtca ggactatgtc aatggcaccg atcaggaaga aattcgaacc ggtgttgatc 301 agtgtatcca gaagtttctg gatattgcaa gacagacaga atgttttttc ttacaaaaaa 361 gattgcagtt atctgtccag aaaccagagc aagttatcaa agaggatgtg tcagaactaa 421 ggaatgaatt acagcggaaa gatgcactag tccagaagca cttgacaaag ctgaggcatt 481 ggcagcaggt gctggaggac atcaacgtgc agcacaaaaa gcccgccgac atccctcagg 541 gctccttggc ctacctggag caggcatctg ccaacatccc tgcacctctg aagccaacgt 601 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 661 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 721 ggatta