Construct: ORF ccsbBroad304_04224
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011704.1_s304c1, BRDN0000404813
- Derived from:
- ccsbBroadEn_04224
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INTS5 (80789)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04224
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80789 | INTS5 | integrator complex subunit 5 | NM_030628.2 | 100% | 100% | |
| 2 | mouse | 109077 | Ints5 | integrator complex subunit 5 | NM_176843.3 | 88.6% | 95.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3123
- ORF length:
- 3057
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgcgctgtgc gaccctcccg gggccccagg gccacctggg cctgccccgg 121 ccacccacgg tcccgcgcct ctcagtgctc aggagctgtc ccaggaaatc aaggcttttc 181 tgactggcgt agaccccatt ctgggccacc aactctcagc ccgggaacat gctcgctgtg 241 gtcttctcct gctccgttct ttgccacctg ctcgggctgc tgtgcttgac cacttgagag 301 gtgtctttga tgagagtgtc cgggcccacc tggctgccct ggatgaaacc cctgtggctg 361 gtccacctca cctccgtcca cctccaccct ctcatgtccc tgctggtgga cctggtctag 421 aggatgtggt tcaggaagtg cagcaggtgc tgtctgagtt tatccgggcc aacccaaagg 481 cctgggcacc tgtgattagt gcatggtcca ttgacctcat ggggcaactg agcagcacgt 541 actcaggcca gcaccagcgt gttccccacg ctactggcgc tcttaatgaa ctgctacagc 601 tgtggatggg ttgtagggcc acgcgtacat taatggacat ctatgtgcag tgcctctcgg 661 ctctcattgg tagctgccca gatgcgtgtg tggatgcctt gctggatacc tctgttcagc 721 attctccaca ctttgactgg gttgtggcac atattggctc ctcttttcct ggcaccatca 781 tttcccgggt tctctcctgt ggccttaagg acttttgtgt ccatggtggg gctggaggtg 841 gagctggcag tagtggtgga agctcttctc agaccccctc tacagacccc ttccctggat 901 ctcctgccat tcctgcggag aaacgggtgc ccaagattgc ctcagttgta ggcatcctag 961 gtcacctggc ctcccgccac ggagatagca tccgacggga gctcctgcga atgttccatg 1021 atagcctggc agggggatct ggaggccgca gtggggaccc ctcccttcag gccacggttc 1081 cgttcctact gcagctggca gtcatgtcac cagctttgct gggcactgtc tctggagagc 1141 ttgtggattg cctcaagccc ccagctgtgc tgagccagct gcagcaacac cttcaaggat 1201 tcccccgaga ggagctggac aacatgttga acctggctgt gcacctggtg agccaggcct 1261 ctggggcagg tgcctaccgc ttgctgcagt tcctggtgga cacagctatg cctgcttcgg 1321 tcattaccac ccagggcctg gctgtgccag acaccgtgcg tgaggcttgt gaccggctaa 1381 tccagctgct gctgctgcac ctgcaaaaac tggttcatca ccggggaggg tctcctgggg 1441 aaggggtgct aggcccgccc ccacctcccc gcttggtgcc ctttttagat gcgctcaaaa 1501 accatgttgg agagctgtgt ggagagacgt tacgattgga acggaagcgc ttcctctggc 1561 agcaccagct cttgggcctg ctgtctgtct atacccggcc tagctgtgga cctgaggcct 1621 tgggccatct gctgagccga gcccgaagcc ctgaagagtt gagtttggcc acccagttat 1681 atgcagggct agtggtcagc ctctctggcc tcctgcccct ggctttccga agctgtctgg 1741 ctcgggtgca tgcagggaca ttacagcctc ccttcacggc ccggttcctg cgcaacttgg 1801 cactgctagt agggtgggaa cagcagggtg gcgagggccc tgcagcccta ggggcgcact 1861 ttggggaatc tgcctcagcc catctgtctg acctggctcc tctcctgcta catcctgagg 1921 aggaagtagc tgaagctgct gcctctctcc tggccatttg tccctttcct tctgaagcct 1981 tatccccctc ccagctcctg ggactggtaa gggctggggt gcaccgcttc tttgcctctc 2041 tgaggctgca tggaccccca ggtgtggcct cagcctgtca gcttctcacc cgcctgtctc 2101 agacatcccc agctgggctc aaggctgtcc tgcagctgct ggttgaagga gccttacatc 2161 gaggcaacac agaactgttt ggtgggcaag tagatgggga caatgagact ctctcagttg 2221 tttcagcttc tttggcttct gcctccctgt tggacactaa ccggaggcac actgcagctg 2281 tgccaggtcc tggagggatt tggtcagttt tccatgctgg agtcatcggc cgtggcttaa 2341 agccacccaa gtttgtccag tcacgaaatc agcaggaagt gatctataac acccagagcc 2401 tcctcagcct cctggttcac tgctgcagtg ccccaggggg cactgaatgt ggggaatgct 2461 ggggggcacc catcttgagt ccagaggcag ccaaagcagt ggcagtgacc ttggtggaga 2521 gtgtgtgtcc cgatgcagct ggtgcagagc tggcctggcc ccccgaggaa cacgcccggg 2581 ccaccgtgga gcgggatctc cgcattggcc ggcgcttccg cgaacagccc ctgctctttg 2641 agctgttaaa gctggtagca gctgcacccc cagccctgtg ctactgttcc gtgctgcttc 2701 gggggctgct ggccgccctc ttgggccatt gggaagcctc tcgccaccct gacacgaccc 2761 actccccctg gcacctggag gcatcctgca ccttagtggc tgtcatggct gagggaagcc 2821 tcctgcctcc ggccctgggt aatatgcatg aagtatttag ccaactggca cctttcgagg 2881 tgcgtctgct gctgctcagt gtctggggtt ttctccggga gcatgggccc ttgcctcaga 2941 agttcatctt ccaatcagag cggggtcgct tcattcggga cttctccagg gagggtggag 3001 gtgagggtgg accccatctg gctgtgctgc acagtgtcct ccaccgcaac atcgaccgcc 3061 taggtctttt ctctggccgt ttccaggcac cttcaccgtc cactctcctt cgacagggga 3121 cgtacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 3181 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 3241 tctggatta