Construct: ORF ccsbBroad304_04240
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016863.1_s304c1, BRDN0000409268
- Derived from:
- ccsbBroadEn_04240
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLPB (81570)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04240
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | NM_030813.6 | 100% | 100% | |
| 2 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | XM_005274320.1 | 95.8% | 95.8% | 452_453ins87 |
| 3 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | NM_001258392.3 | 95.7% | 95.7% | 645_646ins90 |
| 4 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | NM_001258393.3 | 91.6% | 91.6% | 452_453ins87;558_559ins90 |
| 5 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | NM_001258394.2 | 84.3% | 80.4% | 0_1ins242;211_317del |
| 6 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | XM_011545289.2 | 51.8% | 51.2% | (many diffs) |
| 7 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | XM_006507488.3 | 86% | 87.8% | (many diffs) |
| 8 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | NM_009191.3 | 82.3% | 84.5% | (many diffs) |
| 9 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | XM_011241702.2 | 71.4% | 75.5% | (many diffs) |
| 10 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | XM_006507489.3 | 60.9% | 64% | (many diffs) |
| 11 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | XM_011241703.2 | 60.7% | 65.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2187
- ORF length:
- 2121
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct ggggtccctg gtgttgagga gaaaagcact ggcgccacgg ctactcctcc 121 ggctgctcag gtccccaacg ctccggggcc atggaggtgc ttccggccgg aatgtgacta 181 ctgggagtct cggggagccg cagtggctga gggtagccac cggggggcgc cctggaacat 241 cgccggcctt gttctccgga cgtggggcag ccaccggggg gcgccaggga ggacgcttcg 301 ataccaaatg cctcgcggct gccacttggg gacgccttcc tggtcccgaa gaaacactcc 361 caggacagga cagctggaac ggggtcccca gcagggccgg actgggcatg tgcgccctgg 421 ccgcagcgct ggtggttcat tgctacagca agagtccgtc caacaaggat gcagccctgt 481 tggaagctgc ccgtgccaac aatatgcaag aagtcagcag gctgttgtca gaaggtgcag 541 atgtcaatgc aaagcacaga cttggctgga cagcactcat ggtggcagcc atcaaccgaa 601 acaacagtgt ggtacaggtc ctgcttgctg ctggggctga tccaaacctt ggagatgatt 661 tcagcagtgt ttacaagact gccaaggaac agggaatcca ttctttggaa gatgggggac 721 aggacggtgc aagccggcac atcacaaacc agtggacaag tgccctggag ttcaggagat 781 ggctaggact ccccgctggc gtcctgatca cccgagagga tgacttcaac aacaggctga 841 acaaccgcgc cagtttcaag ggctgcacgg ccttgcacta tgctgttctt gctgatgact 901 accgcactgt caaggagctg cttgatggag gagccaaccc cctgcagagg aatgaaatgg 961 gacacacacc cttggattat gcccgagaag gggaagtgat gaagcttctg aggacttctg 1021 aagccaagta ccaagagaag cagcggaagc gtgaggctga ggagcggcgc cgcttccccc 1081 tggagcagcg actaaaggag cacatcattg gccaggagag cgccatcgcc acagtgggtg 1141 ctgcgatccg gaggaaggag aatggctggt acgatgaaga acaccctctg gtcttcctct 1201 tcttgggatc atctggaata ggaaaaacag agctggccaa gcagacagcc aaatatatgc 1261 acaaagatgc taaaaagggc ttcatcaggc tggacatgtc cgagttccag gagcgacacg 1321 aggtggccaa gtttattggg tctccaccag gctacgttgg ccatgaggag ggtggccagc 1381 tgaccaagaa gttgaagcag tgccccaatg ctgtggtgct ctttgatgaa gtagacaagg 1441 cccatccaga tgtgctcacc atcatgctgc agctgtttga tgagggccgg ctgacagatg 1501 gaaaagggaa gaccattgat tgcaaggacg ccatcttcat catgacctcc aatgtggcca 1561 gcgacgagat cgcacagcac gcgctgcagc tgaggcagga agctttggag atgagccgta 1621 accgtattgc cgaaaacctg ggggatgtcc agataagtga caagatcacc atctcaaaga 1681 acttcaagga gaatgtgatt cgccctatcc tgaaagctca cttccggagg gatgagtttc 1741 tgggacggat caatgagatc gtctacttcc tccccttctg ccactcggag ctcatccaac 1801 tcgtcaacaa ggaactaaac ttctgggcca agagagccaa gcaaaggcac aacatcacgc 1861 tgctctggga ccgcgaggtg gcagatgtgc tggtcgacgg ctacaatgtg cactatggcg 1921 cccgctccat caaacatgag gtagaacgcc gtgtggtgaa ccagctggca gcagcctatg 1981 agcaggacct gctgccaggg ggctgtactt tgcgcatcac ggtggaggac tcagacaagc 2041 agctactcaa aagcccagaa ctgccctcac cccaggctga gaagcgcctc cccaagctgc 2101 gtctggagat catcgacaag gacagcaaga ctcgcagact ggacatccgg gcaccactgc 2161 accctgagaa ggtgtgcaac accatctacc caactttctt gtacaaagtg gttggtaagc 2221 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2281 ggcgcgttaa gtcgacaatc aacctctgga tta