Construct: ORF ccsbBroad304_04245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000926.1_s304c1, BRDN0000401367
- Derived from:
- ccsbBroadEn_04245
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LBH (81606)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04245
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81606 | LBH | LBH regulator of WNT signal... | NM_030915.4 | 100% | 100% | |
| 2 | human | 81606 | LBH | LBH regulator of WNT signal... | XM_017005059.1 | 42.1% | 42.3% | 1_375del;376_377ins24 |
| 3 | mouse | 77889 | Lbh | limb-bud and heart | NM_029999.4 | 89.5% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 384
- ORF length:
- 315
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctatatat ttccccattc actgccccga ctatctgaga tcggccaaga 121 tgactgaggt gatgatgaac acccagccca tggaggagat cggcctcagc ccccgcaagg 181 atggcctttc ctaccagatc ttcccagacc cgtcagattt tgaccgctgc tgcaaactga 241 aggaccgtct gccctccata gtggtggaac ccacagaagg ggaggtggag agcggggagc 301 tccggtggcc ccctgaggag ttcctggtcc aggaggatga gcaagataac tgcgaagaga 361 cagcgaaaga aaataaagag cagttgccaa ctttcttgta caaagtggtt ggtaagccta 421 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 481 gcgttaagtc gacaatcaac ctctggatta