Construct: ORF ccsbBroad304_04252
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014102.1_s304c1, BRDN0000402675
- Derived from:
- ccsbBroadEn_04252
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP1LC3B (81631)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04252
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81631 | MAP1LC3B | microtubule associated prot... | NM_022818.5 | 100% | 100% | |
| 2 | human | 643246 | MAP1LC3B2 | microtubule associated prot... | NM_001085481.3 | 99.4% | 99.2% | 30G>C;338G>A |
| 3 | mouse | 67443 | Map1lc3b | microtubule-associated prot... | NM_026160.4 | 85.1% | 95.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtcggagaag accttcaagc agcgccgcac cttcgaacaa agagtagaag 121 atgtccgact tattcgagag cagcatccaa ccaaaatccc ggtgataata gaacgataca 181 agggtgagaa gcagcttcct gttctggata aaacaaagtt ccttgtacct gaccatgtca 241 acatgagtga gctcatcaag ataattagaa ggcgcttaca gctcaatgct aatcaggcct 301 tcttcctgtt ggtgaacgga cacagcatgg tcagcgtctc cacaccaatc tcagaggtgt 361 atgagagtga gaaagatgaa gatggattcc tgtacatggt ctatgcctcc caggagacgt 421 tcgggatgaa attgtcagtg tacccaactt tcttgtacaa agtggttggt aagcctatcc 481 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 541 ttaagtcgac aatcaacctc tggatta