Construct: ORF ccsbBroad304_04256
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003116.1_s304c1, BRDN0000398957
- Derived from:
- ccsbBroadEn_04256
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF146 (81847)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04256
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81847 | RNF146 | ring finger protein 146 | NM_001242844.1 | 100% | 100% | |
| 2 | human | 81847 | RNF146 | ring finger protein 146 | NM_001242845.1 | 100% | 100% | |
| 3 | human | 81847 | RNF146 | ring finger protein 146 | NM_001242846.1 | 100% | 100% | |
| 4 | human | 81847 | RNF146 | ring finger protein 146 | NM_001242847.1 | 100% | 100% | |
| 5 | human | 81847 | RNF146 | ring finger protein 146 | NM_001242848.1 | 100% | 100% | |
| 6 | human | 81847 | RNF146 | ring finger protein 146 | NM_001242852.1 | 100% | 100% | |
| 7 | human | 81847 | RNF146 | ring finger protein 146 | NM_030963.3 | 100% | 100% | |
| 8 | human | 81847 | RNF146 | ring finger protein 146 | XM_006715571.4 | 100% | 100% | |
| 9 | human | 81847 | RNF146 | ring finger protein 146 | XM_011536161.3 | 100% | 100% | |
| 10 | human | 81847 | RNF146 | ring finger protein 146 | XM_011536162.3 | 100% | 100% | |
| 11 | human | 81847 | RNF146 | ring finger protein 146 | XM_011536163.3 | 100% | 100% | |
| 12 | human | 81847 | RNF146 | ring finger protein 146 | XM_011536164.3 | 100% | 100% | |
| 13 | human | 81847 | RNF146 | ring finger protein 146 | XM_017011338.2 | 100% | 100% | |
| 14 | human | 81847 | RNF146 | ring finger protein 146 | XM_017011339.2 | 100% | 100% | |
| 15 | human | 81847 | RNF146 | ring finger protein 146 | XM_017011340.2 | 100% | 100% | |
| 16 | human | 81847 | RNF146 | ring finger protein 146 | XM_017011341.2 | 100% | 100% | |
| 17 | human | 81847 | RNF146 | ring finger protein 146 | XM_017011342.2 | 100% | 100% | |
| 18 | human | 81847 | RNF146 | ring finger protein 146 | XM_017011343.2 | 100% | 100% | |
| 19 | human | 81847 | RNF146 | ring finger protein 146 | XM_024446563.1 | 100% | 100% | |
| 20 | human | 81847 | RNF146 | ring finger protein 146 | XM_024446564.1 | 100% | 100% | |
| 21 | human | 81847 | RNF146 | ring finger protein 146 | NM_001242849.1 | 99.7% | 99.7% | 1_3delATG |
| 22 | human | 81847 | RNF146 | ring finger protein 146 | NM_001242850.1 | 99.7% | 99.7% | 1_3delATG |
| 23 | human | 81847 | RNF146 | ring finger protein 146 | NM_001242851.1 | 99.7% | 99.7% | 1_3delATG |
| 24 | human | 81847 | RNF146 | ring finger protein 146 | XM_017011336.2 | 99.7% | 99.7% | 1_3delATG |
| 25 | mouse | 68031 | Rnf146 | ring finger protein 146 | NM_001110196.1 | 89.1% | 92.2% | (many diffs) |
| 26 | mouse | 68031 | Rnf146 | ring finger protein 146 | NM_001110197.1 | 89.1% | 92.2% | (many diffs) |
| 27 | mouse | 68031 | Rnf146 | ring finger protein 146 | NM_001110198.1 | 89.1% | 92.2% | (many diffs) |
| 28 | mouse | 68031 | Rnf146 | ring finger protein 146 | NM_001284279.1 | 89.1% | 92.2% | (many diffs) |
| 29 | mouse | 68031 | Rnf146 | ring finger protein 146 | NM_026518.4 | 89.1% | 92.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1140
- ORF length:
- 1074
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tggctgtggt gaaattgatc attcaataaa catgcttcct acaaacagga 121 aagcgaacga gtcctgttct aatactgcac cttctttaac cgtccctgaa tgtgccattt 181 gtctgcaaac atgtgttcat ccagtcagtc tgccctgtaa gcacgttttc tgctatctat 241 gtgtaaaagg agcttcatgg cttggaaagc ggtgtgctct ttgtcgacaa gaaattcccg 301 aggatttcct tgacaagcca accttgttgt caccagaaga actcaaggca gcaagtagag 361 gaaatggtga atatgcatgg tattatgaag gaagaaatgg gtggtggcag tacgatgagc 421 gcactagtag agagctggaa gatgcttttt ccaaaggtaa aaagaacact gaaatgttaa 481 ttgctggctt tctgtatgtc gctgatcttg aaaacatggt tcaatatagg agaaatgaac 541 atggacgtcg caggaagatt aagcgagata taatagatat accaaagaag ggagtagctg 601 gacttaggct agactgtgat gctaataccg taaacctagc aagagagagc tctgctgacg 661 gagcggacag tgtatcagca cagagtggag cttctgttca gcccctagtg tcttctgtaa 721 ggcccctaac atcagtagat ggtcagttaa caagccctgc aacaccatcc cctgatgcaa 781 gcacttctct ggaagactct tttgctcatt tacaactcag tggagacaac acagctgaaa 841 ggagtcatag gggagaagga gaagaagatc atgaatcacc atcttcaggc agggtaccag 901 caccagacac ctccattgaa gaaactgaat cagatgccag tagtgatagt gaggatgtat 961 ctgcagttgt tgcacagcac tccttgaccc aacagagact tttggtttct aatgcaaacc 1021 agacagtacc cgatcgatca gatcgatcgg gaactgatcg atcagtagca gggggtggaa 1081 cagtgagtgt cagtgtcaga tctagaaggc ctgatggaca gtgcacagta actgaagttt 1141 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1201 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1261 ggatta