Construct: ORF ccsbBroad304_04336
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013888.1_s304c1, BRDN0000402079
- Derived from:
- ccsbBroadEn_04336
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R1B (84152)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04336
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84152 | PPP1R1B | protein phosphatase 1 regul... | NM_001242464.2 | 100% | 100% | |
| 2 | human | 84152 | PPP1R1B | protein phosphatase 1 regul... | NM_181505.4 | 100% | 100% | |
| 3 | human | 84152 | PPP1R1B | protein phosphatase 1 regul... | XM_017025217.2 | 100% | 100% | |
| 4 | human | 84152 | PPP1R1B | protein phosphatase 1 regul... | NM_032192.4 | 82.3% | 82.3% | 1_108del |
| 5 | human | 84152 | PPP1R1B | protein phosphatase 1 regul... | XM_017025216.2 | 82.3% | 82.3% | 1_108del |
| 6 | mouse | 19049 | Ppp1r1b | protein phosphatase 1, regu... | NM_001313970.1 | 79.8% | 79.2% | (many diffs) |
| 7 | mouse | 19049 | Ppp1r1b | protein phosphatase 1, regu... | NM_144828.2 | 65.9% | 65.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gttccggctc tcagagcact cctcaccaga ggaggaagcc tccccccacc 121 agagagcctc aggagagggg caccatctca agtcgaagag acccaacccc tgtgcctaca 181 caccaccttc gctgaaagct gtgcagcgca ttgctgagtc tcacctgcag tctatcagca 241 atttgaatga gaaccaggcc tcagaggagg aggatgagct gggggagctt cgggagctgg 301 gttatccaag agaggaagat gaggaggaag aggaggatga tgaagaagag gaagaagaag 361 aggacagcca ggctgaagtc ctgaaggtca tcaggcagtc tgctgggcaa aagacaacct 421 gtggccaggg tctggaaggg ccctgggagc gcccaccccc tctggatgag tccgagagag 481 atggaggctc tgaggaccaa gtggaagacc cagcactaag tgagcctggg gaggaacctc 541 agcgcccttc cccctctgag cctggcacat acccaacttt cttgtacaaa gtggttggta 601 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 661 ggtggcgcgt taagtcgaca atcaacctct ggatta