Construct: ORF ccsbBroad304_04337
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009448.1_s304c1, BRDN0000461736
- Derived from:
- ccsbBroadEn_04337
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASCC2 (84164)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04337
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369921.1 | 100% | 100% | |
| 2 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369922.1 | 100% | 100% | |
| 3 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369923.1 | 100% | 100% | |
| 4 | human | 84164 | ASCC2 | activating signal cointegra... | NM_032204.5 | 100% | 100% | |
| 5 | human | 84164 | ASCC2 | activating signal cointegra... | XM_017028998.1 | 100% | 100% | |
| 6 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369924.1 | 99.4% | 99.4% | 1786_1787insAGGTGCCACTGC |
| 7 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369920.1 | 99.3% | 99.3% | 2021_2035del |
| 8 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530442.3 | 99.3% | 99.3% | 2021_2035del |
| 9 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530443.2 | 99.3% | 99.3% | 2021_2035del |
| 10 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530444.2 | 99.3% | 99.3% | 2021_2035del |
| 11 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530445.2 | 99.3% | 99.3% | 2021_2035del |
| 12 | human | 84164 | ASCC2 | activating signal cointegra... | XM_017028996.1 | 99.3% | 99.3% | 2021_2035del |
| 13 | human | 84164 | ASCC2 | activating signal cointegra... | XM_017028992.1 | 93.3% | 93.3% | 1_162del |
| 14 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369926.1 | 92.9% | 92.9% | 80_81ins159 |
| 15 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369927.1 | 92.9% | 92.9% | 80_81ins159 |
| 16 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369928.1 | 92.9% | 92.9% | 80_81ins159 |
| 17 | human | 84164 | ASCC2 | activating signal cointegra... | XM_024452287.1 | 92.7% | 92.7% | 1_162del;2183_2197del |
| 18 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369929.1 | 92.4% | 92.4% | 80_81ins159;1627_1628insAGGTGCCACTGC |
| 19 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369925.1 | 92.3% | 92.3% | 80_81ins159;1862_1876del |
| 20 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530448.2 | 92.3% | 92.3% | 80_81ins159;1862_1876del |
| 21 | human | 84164 | ASCC2 | activating signal cointegra... | XM_024452289.1 | 90.2% | 90.2% | 1_162del;995_996ins75 |
| 22 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001242906.2 | 89.9% | 89.9% | 80_81ins159;252_253ins69 |
| 23 | human | 84164 | ASCC2 | activating signal cointegra... | XM_024452288.1 | 89.7% | 89.7% | 1_162del;995_996ins75;2108_2122del |
| 24 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369930.1 | 89.6% | 89.6% | 80_81ins159;674_675ins75 |
| 25 | human | 84164 | ASCC2 | activating signal cointegra... | XM_024452291.1 | 86.8% | 86.8% | 1_162del;242_243ins159 |
| 26 | human | 84164 | ASCC2 | activating signal cointegra... | XM_024452290.1 | 86.2% | 86.2% | 1_162del;242_243ins159;2024_2038del |
| 27 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369931.1 | 84.9% | 84.9% | 0_1ins342 |
| 28 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369932.1 | 84.9% | 84.9% | 0_1ins342 |
| 29 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369933.1 | 84.9% | 84.9% | 0_1ins342 |
| 30 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369934.1 | 84.9% | 84.9% | 0_1ins342 |
| 31 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369935.1 | 84.9% | 84.9% | 0_1ins342 |
| 32 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369936.1 | 84.9% | 84.9% | 0_1ins342 |
| 33 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369937.1 | 84.9% | 84.9% | 0_1ins342 |
| 34 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369938.1 | 84.9% | 84.9% | 0_1ins342 |
| 35 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369939.1 | 84.9% | 84.9% | 0_1ins342 |
| 36 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369940.1 | 84.9% | 84.9% | 0_1ins342 |
| 37 | human | 84164 | ASCC2 | activating signal cointegra... | XM_017029005.1 | 84.9% | 84.9% | 0_1ins342 |
| 38 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369941.1 | 84.4% | 84.4% | 0_1ins342;1444_1445insAGGTGCCACTGC |
| 39 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530450.2 | 84.3% | 84.3% | 0_1ins342;1679_1693del |
| 40 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530451.2 | 84.3% | 84.3% | 0_1ins342;1679_1693del |
| 41 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530452.2 | 84.3% | 84.3% | 0_1ins342;1679_1693del |
| 42 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530453.2 | 84.3% | 84.3% | 0_1ins342;1679_1693del |
| 43 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530454.2 | 84.3% | 84.3% | 0_1ins342;1679_1693del |
| 44 | human | 84164 | ASCC2 | activating signal cointegra... | XM_011530455.2 | 84.3% | 84.3% | 0_1ins342;1679_1693del |
| 45 | human | 84164 | ASCC2 | activating signal cointegra... | XM_024452292.1 | 84.3% | 84.3% | 0_1ins342;1679_1693del |
| 46 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369942.1 | 76.4% | 76.4% | 0_1ins534 |
| 47 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369943.1 | 68.8% | 68.8% | 0_1ins708 |
| 48 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369944.1 | 68.8% | 68.8% | 0_1ins708 |
| 49 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369945.1 | 68.8% | 68.8% | 0_1ins708 |
| 50 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369946.1 | 68.8% | 68.8% | 0_1ins708 |
| 51 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369947.1 | 68.8% | 68.8% | 0_1ins708 |
| 52 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369948.1 | 68.8% | 68.8% | 0_1ins708 |
| 53 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369949.1 | 68.8% | 68.8% | 0_1ins708 |
| 54 | human | 84164 | ASCC2 | activating signal cointegra... | NM_001369950.1 | 68.5% | 68.5% | 0_1ins708;978_979insAGCACC |
| 55 | mouse | 75452 | Ascc2 | activating signal cointegra... | NM_029291.1 | 85.8% | 86.8% | (many diffs) |
| 56 | mouse | 75452 | Ascc2 | activating signal cointegra... | XM_006514874.3 | 72% | 73.2% | (many diffs) |
| 57 | mouse | 75452 | Ascc2 | activating signal cointegra... | XR_380985.3 | 61.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2337
- ORF length:
- 2271
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc agctctgccc ctggaccaac tccagatcac ccacaaggac ccgaagacag 121 gaaagctgag gacttcacca gcgctgcacc ccgagcagaa ggcagaccgg tattttgtgt 181 tatacaaacc gccccctaaa gacaacattc ccgccctagt ggaggagtac ctggaacgcg 241 ccaccttcgt agccaatgac ctcgactggc tcctggcctt gcctcacgat aaattctggt 301 gccaggtgat ctttgacgag actctacaga agtgcctgga ctcctacctg cgctatgtcc 361 cccgcaaatt cgacgagggg gtggcctcag cccctgaggt tgttgacatg cagaagcgcc 421 tccatcgaag tgtttttctc accttcctcc gcatgtccac tcacaaggaa tccaaagatc 481 acttcatttc cccttctgcg tttggagaaa tcctctacaa taacttcctc tttgacattc 541 caaagatcct ggacctctgc gtgctctttg gaaaaggcaa ctcaccactg ctccagaaga 601 tgataggaaa catctttaca cagcagccaa gttactacag tgacctggat gaaaccctgc 661 ctaccatcct tcaggtcttc agcaatatcc tccagcactg tggtttgcaa ggggacgggg 721 ccaataccac accccagaag cttgaggaga ggggccgatt gacccccagt gacatgcctc 781 tcctggaatt aaaggacatt gttctctacc tttgtgatac ctgcaccaca ctttgggcct 841 ttctggatat cttccctttg gcttgccaga ccttccagaa gcacgacttt tgttacagac 901 tagcttcctt ctacgaagca gcaattcccg aaatggagtc tgcaattaag aagaggaggc 961 ttgaagatag caagcttctt ggtgacctgt ggcagaggct ctcccattcc aggaagaagc 1021 taatggagat tttccacatc atcctgaacc agatctgcct ccttcccatc ctagaaagca 1081 gctgtgacaa cattcagggc ttcatcgaag agttccttca gatcttcagc tccttgctgc 1141 aggagaagag gttcctccgg gactatgatg cactcttccc cgtggccgaa gacatcagct 1201 tgctgcagca ggcctcatca gtcttggacg agacgcggac tgcctacatc ctccaggcag 1261 tcgagagtgc atgggaaggg gtggacagac ggaaagccac agatgctaaa gacccatcgg 1321 tgattgagga gcctaatggg gagcctaacg gggtcacggt gacagcagag gcagtcagtc 1381 aagcatcatc acatccggag aactcggagg aagaggagtg catgggagca gccgcggctg 1441 tgggccctgc catgtgtggg gtggaactgg actctctcat ctcccaagtg aaggacctgc 1501 tgccagacct tggtgagggc ttcatcctgg cctgcctgga gtactaccac tacgacccag 1561 agcaggtgat caacaatatc ctggaggagc ggctggcccc caccctcagc cagctggacc 1621 gcaacctaga cagagaaatg aaaccagacc ctacacccct gctgacgtct cgccacaacg 1681 tcttccagaa tgacgagttt gatgtgttca gcagggactc agtagacctg agccgggtgc 1741 acaagggcaa gagcaccagg aaggaggaaa acacgcggag tttgctgaac gacaagcgtg 1801 cagtggcggc acagcggcag cgctacgagc agtacagcgt ggtggtggag gaggtgccac 1861 tgcagccagg cgagagcctg ccctaccaca gtgtctacta cgaggatgag tacgatgaca 1921 catacgatgg caaccaggtg ggcgccaatg atgcagactc tgatgacgag ctcatcagcc 1981 gcaggccatt caccatccct caggtgctga gaaccaaagt gcctagagaa gggcaggagg 2041 aggatgacga cgatgaggaa gacgatgctg acgaggaggc tcccaagccc gaccattttg 2101 ttcaggaccc tgcagtgctg agagagaagg cagaagccag gcgcatggcc tttctcgcca 2161 agaaagggta ccggcatgac agctcaacag cagtggccgg cagcccccga ggccatgggc 2221 agagccgcga gacaacccag gaacgcagga agaaggaagc caacaaggcg acaagagcca 2281 accacaaccg gagaaccatg gccgaccgca agaggagcaa aggcatgatc ccatcctgcc 2341 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2401 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2461 tta