Transcript: Mouse XM_006514874.3

PREDICTED: Mus musculus activating signal cointegrator 1 complex subunit 2 (Ascc2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ascc2 (75452)
Length:
4915
CDS:
387..2294

Additional Resources:

NCBI RefSeq record:
XM_006514874.3
NBCI Gene record:
Ascc2 (75452)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006514874.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000247898 TGGCCTTGCCTCACGATAAAT pLKO_005 253 5UTR 100% 15.000 21.000 N Ascc2 n/a
2 TRCN0000247895 ATGACTATGAGGACGAGTATG pLKO_005 1852 CDS 100% 10.800 15.120 N Ascc2 n/a
3 TRCN0000247897 CTCCGTGACTACGACACATTC pLKO_005 1134 CDS 100% 10.800 15.120 N Ascc2 n/a
4 TRCN0000179687 GTTGTCATCTCGTCACAACAT pLKO.1 1634 CDS 100% 0.495 0.396 N Ascc2 n/a
5 TRCN0000247896 GCAGAAGGCAGACCGGTATTT pLKO_005 134 5UTR 100% 13.200 9.240 N Ascc2 n/a
6 TRCN0000247899 GTGAGCGCCTTCTTGTCAAAC pLKO_005 2371 3UTR 100% 10.800 7.560 N Ascc2 n/a
7 TRCN0000073314 GCAATTAAGAAGAGGAGGCTT pLKO.1 921 CDS 100% 2.640 1.848 N ASCC2 n/a
8 TRCN0000183940 CCGAAAGTTTGATGAGTGGGT pLKO.1 341 5UTR 100% 0.660 0.462 N Ascc2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006514874.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04337 pDONR223 100% 72% 73.2% None (many diffs) n/a
2 ccsbBroad304_04337 pLX_304 0% 72% 73.2% V5 (many diffs) n/a
3 TRCN0000476910 CTAGCCAATTTGCCAAATGCCACC pLX_317 8.3% 72% 73.2% V5 (many diffs) n/a
Download CSV