Construct: ORF ccsbBroad304_04468
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016196.1_s304c1, BRDN0000409715
- Derived from:
- ccsbBroadEn_04468
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM128 (85013)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04468
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85013 | TMEM128 | transmembrane protein 128 | NM_032927.3 | 100% | 100% | |
| 2 | human | 85013 | TMEM128 | transmembrane protein 128 | XM_024454263.1 | 100% | 100% | |
| 3 | human | 85013 | TMEM128 | transmembrane protein 128 | NM_001297551.2 | 80.9% | 78.8% | (many diffs) |
| 4 | human | 85013 | TMEM128 | transmembrane protein 128 | NM_001297552.2 | 80.9% | 78.8% | (many diffs) |
| 5 | human | 85013 | TMEM128 | transmembrane protein 128 | XM_005248034.3 | 80.9% | 78.8% | (many diffs) |
| 6 | mouse | 66309 | Tmem128 | transmembrane protein 128 | NM_025480.3 | 69.5% | 70.4% | (many diffs) |
| 7 | mouse | 66309 | Tmem128 | transmembrane protein 128 | XM_006504046.2 | 69.5% | 70.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 489
- ORF length:
- 423
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gccatttggg tacaaaacag aaacctccac agctgttgag aaaaaggaga 121 aacctcttcc aagacttaat atccattctg gattctggat tttggcatcc attgttgtga 181 cctattatgt tgacttcttt aaaaccctta aagaaaactt ccacactagc agctggtttc 241 tctgtggcag tgccttgttg cttgtcagtt tatcaattgc attttactgc atagtctacc 301 tggaatggta ttgtggaatt ggagaatatg atgtcaagta tccagccttg atacccatta 361 ccactgcctc ctttattgca gcaggaattt gcttcaacat tgctttatgg catgtgtggt 421 cgtttttcac tccattgttg ttgtttaccc agtttatggg ggttgtcatg tttatcacac 481 tccttggatg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 541 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 601 tcaacctctg gatta