Construct: ORF ccsbBroad304_04530
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015131.1_s304c1, BRDN0000410427
- Derived from:
- ccsbBroadEn_04530
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP95 (90799)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04530
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90799 | CEP95 | centrosomal protein 95 | NM_138363.3 | 100% | 100% | |
| 2 | human | 90799 | CEP95 | centrosomal protein 95 | XM_005257779.3 | 93.6% | 91.5% | (many diffs) |
| 3 | human | 90799 | CEP95 | centrosomal protein 95 | NR_133644.2 | 88.6% | (many diffs) | |
| 4 | human | 90799 | CEP95 | centrosomal protein 95 | XR_001752675.1 | 82.6% | 1_338del;1878_1881delGTGG;2806_2980del | |
| 5 | human | 90799 | CEP95 | centrosomal protein 95 | NM_001316990.2 | 80% | 80% | 0_1ins492 |
| 6 | human | 90799 | CEP95 | centrosomal protein 95 | XM_024451020.1 | 77.9% | 73.9% | (many diffs) |
| 7 | human | 90799 | CEP95 | centrosomal protein 95 | XM_005257780.2 | 74.2% | 72% | (many diffs) |
| 8 | human | 90799 | CEP95 | centrosomal protein 95 | XM_017025287.1 | 74.2% | 72% | (many diffs) |
| 9 | human | 90799 | CEP95 | centrosomal protein 95 | XM_006722166.4 | 67.4% | 63.1% | (many diffs) |
| 10 | human | 90799 | CEP95 | centrosomal protein 95 | XM_017025288.2 | 67.4% | 63.1% | (many diffs) |
| 11 | human | 90799 | CEP95 | centrosomal protein 95 | XR_002958083.1 | 67.3% | 1_338del;1489_1490ins154;2225_2226ins422 | |
| 12 | human | 90799 | CEP95 | centrosomal protein 95 | XR_002958084.1 | 60.8% | (many diffs) | |
| 13 | human | 90799 | CEP95 | centrosomal protein 95 | XM_011525458.2 | 53.5% | 46.8% | 1151_1152ins154;1320_1321ins989 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2529
- ORF length:
- 2463
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc aggctcggat gctgagtggg taaccattgc caataacctt ctttttaagt 121 gtcatataca tctgagaata catgaacttc aagactgtga tgctaatgtt tttattgctc 181 tttatcagtc tattttggga gaaaaggtac cagacctcat agttattcct aggagtcaag 241 aagatgatgc acacaatgta caagcagtaa ttgattcact ggccttggac tacttgcagg 301 tcagcttgtc tcacataaca ggagaaaata tagtgaaagg agataaagaa tctattaaga 361 atctcctgga aatatttgat ggtttgttgg agtatcttac agaacgcatc agtgaaacat 421 ctcatgagaa aagtgaaact gaacagtatt ttaaagaatc tgatcgagga gaacgtttgg 481 aagagccaga aagtactaaa gaatctaaat catcatggaa aagagtttct tttgggaggt 541 gctccttgtc ttctgagatg ttgggcccct cttgggatgg agatgaagca gaatccactg 601 gtgaaatcat tagacttgga gacacagcac acaccttttc tctaagaagt aatggtgctc 661 aatgtcctaa tgaaatgctg tctaaaaaag ccttagcctc accaagttct aaatcacatg 721 aagatatgtt gtaccctcct agtgttttgt ccaagagtag gacatccttt gttgaagaca 781 cggaaaccct ttctgtgagt gggattccaa atgctaggaa gctaggggag cctatccgag 841 cagctattcc tttacatcca ccctaccacc cttcagagcc tcgagcaccc tgccccatag 901 gaaaagaata cttgcattca agtcactgct ccccagccgt aaattctact ggagagcata 961 cggaattttc tggggatcta gatgatggac ttttcttaat ttccaagttg cctaaaggca 1021 gcaaatggga agtatatcca gctcaggtcc aagggcctag gacaaggaag cctcccaaag 1081 gaaaaagaaa tgaaaacaga gctacagcct catcctgcaa ttcacctttc ccccagaggc 1141 caagaaagag attaacagaa caagaattac atgatgtatc agaaaaactc tctcagcggc 1201 tttctgaact agattggatg ttaaaaagtg ctctgggtga tcggattaaa gaaaagactg 1261 accataaaga agaaaatact ggaaatgagg aggtagagga tggaactgag gagacactgt 1321 ctcagcacag tgatggcatc gtggagtatg ggccaaagaa gtcaaggcca ggactttcca 1381 tgcgtagaaa gccaccctac agatcccatt cgctctctcc atctccagtt aacaaacaca 1441 aacagttcca cttggagaga aaaaggcagc gcaagccaag agaaacagat gtccgccaat 1501 tccaagcaca ggcgtttact gaagcatttg aaagggaact aagaagacat aaagttcaag 1561 agaatattgg acctctaaga atacatgaga aggaggagga aacagaaaaa atatacagag 1621 gagaagctgt tcgtaaagga actccagaat gtagtcagcc ctggaagatt tactctagaa 1681 aaaccacaac gcagagtcta agaggtggcc tcccaaagcc aaataaagca gttccaatga 1741 aagtaagtga acacagtctc ctgcccctta tgctggagca gtttccgttt ctttatgttt 1801 ctggcccaac actaagcaaa atgtggaaac agcaaattgc acaggttgaa cagcttaaga 1861 aagaagcatg tagagaaaat cgatcaaaga agaaactcca agatgaaata gaagaagccc 1921 taagaaggca tgacctcctt actacccttg tcaagaaaga atatgaacat aacaagagac 1981 tgcaagactt caaggactgc attcgtaggc aaaggttgac ccaatcaaag ataaaagaaa 2041 atcgacagca aatcgttcgt gctcgaaaat attatgatga ttatagagtt cagttgtgtg 2101 caaaaatgat gagaatgagg acccgggaag aaatgatatt taagaaactg tttgaagaag 2161 gtttaaacat tcaaaagcaa agattacgag acctaagaaa ctatgccaaa gaaaagcgag 2221 atgaacaaag gagacgccac caggatgaac tggactccat ggagaactac tataaggacc 2281 agttttcatt gctggcagaa gccatatcac aggaacatca agaacttaaa gccagagaga 2341 aatctcaggc ccagacatta cataaggtga agagggagct gagatctaag atggagaagg 2401 aaattcagca gctgcaggac atgataacac agaatgatga tgatgttttc ttccgggaac 2461 tggaagctga gcgcttcaga tctcggcttc agctggcttc ctttcagtac agtaaaagtc 2521 cctccctatg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 2581 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 2641 tcaacctctg gatta