Construct: ORF ccsbBroad304_04804
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002688.1_s304c1, BRDN0000460283
- Derived from:
- ccsbBroadEn_04804
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MICOS13 (125988)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04804
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 125988 | MICOS13 | mitochondrial contact site ... | NM_205767.2 | 100% | 100% | |
| 2 | human | 125988 | MICOS13 | mitochondrial contact site ... | NM_001308240.1 | 81.9% | 79.2% | (many diffs) |
| 3 | human | 125988 | MICOS13 | mitochondrial contact site ... | NM_001365761.1 | 81.9% | 79.2% | (many diffs) |
| 4 | human | 125988 | MICOS13 | mitochondrial contact site ... | XM_011527675.2 | 60.4% | 57.8% | (many diffs) |
| 5 | mouse | 224904 | 2410015M20Rik | RIKEN cDNA 2410015M20 gene | NM_153152.3 | 84.3% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 423
- ORF length:
- 354
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcacnat ggtggcccgg gtgtggtcgc tgatgaggtt cctcatcaag ggaagtgtgg 121 ctgggggcgc cgtctacctg gtgtacgacc aggagctgct ggggcccagc gacaagagcc 181 aggcagccct acagaaggct ggggaggtgg tcccccccgc catgtaccag ttcagccagt 241 acgtgtgtca gcagacaggc ctgcagatac cccagctccc agcccctcca aagatttact 301 ttcccatccg tgactcctgg aatgcaggca tcatgacggt gatgtcagct ctgtcggtgg 361 ccccctccaa ggcccgcgag tactccaagg agggctggga gtatgtgaag gcgcgcacca 421 agttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 481 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 541 tctggatta