Construct: ORF ccsbBroad304_04832
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000609.1_s304c1, BRDN0000401576
- Derived from:
- ccsbBroadEn_04832
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT2 (127833)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04832
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127833 | SYT2 | synaptotagmin 2 | NM_001136504.1 | 100% | 100% | |
| 2 | human | 127833 | SYT2 | synaptotagmin 2 | NM_177402.5 | 100% | 100% | |
| 3 | human | 127833 | SYT2 | synaptotagmin 2 | XM_017000313.1 | 100% | 100% | |
| 4 | human | 127833 | SYT2 | synaptotagmin 2 | XM_011509192.2 | 99.2% | 99.2% | 343_351delCAGCTCCCA |
| 5 | human | 127833 | SYT2 | synaptotagmin 2 | XM_017000311.2 | 99.2% | 99.2% | 343_351delCAGCTCCCA |
| 6 | human | 127833 | SYT2 | synaptotagmin 2 | XM_017000312.1 | 99.2% | 99.2% | 343_351delCAGCTCCCA |
| 7 | human | 127833 | SYT2 | synaptotagmin 2 | XM_017000310.2 | 88% | 88% | 1_171del |
| 8 | human | 127833 | SYT2 | synaptotagmin 2 | XM_017000309.2 | 87.4% | 87.4% | 1_171del;514_522delCAGCTCCCA |
| 9 | mouse | 20980 | Syt2 | synaptotagmin II | NM_009307.3 | 88.7% | 97.6% | (many diffs) |
| 10 | mouse | 20980 | Syt2 | synaptotagmin II | XM_006529322.1 | 88.7% | 97.6% | (many diffs) |
| 11 | mouse | 20980 | Syt2 | synaptotagmin II | XM_006529323.3 | 88.7% | 97.6% | (many diffs) |
| 12 | mouse | 20980 | Syt2 | synaptotagmin II | XM_006529324.3 | 52.9% | 59.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1326
- ORF length:
- 1257
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggaacatt ttcaagagga accaggagcc tattgtggct cctgccacca 121 ccaccgccac gatgcccatt ggacccgtgg acaactccac tgagagtggg ggtgctgggg 181 agagccagga ggacatgttt gccaaactga aggagaagtt attcaatgag ataaacaaga 241 ttcccttacc accctgggca ctgatcgcca ttgctgtggt tgctgggctc ctgcttctca 301 cctgctgctt ctgcatctgc aagaaatgct gctgcaagaa gaagaagaac aagaaggaga 361 agggcaaagg catgaagaat gccatgaaca tgaaggacat gaaagggggt caggatgacg 421 acgacgcaga gacaggcctg actgaggggg aaggtgaagg ggaggaggag aaagagccag 481 agaacctggg caaactgcag ttttccctgg actatgattt tcaggctaat cagcttactg 541 tgggcgttct gcaggctgct gaactgcctg ccctggacat gggaggcacc tcagaccctt 601 atgtcaaggt cttcctcctt cctgacaaga agaagaaata tgagaccaaa gtccatcgga 661 agacactgaa ccctgccttc aatgaaacct tcaccttcaa ggtgccatac caggagcttg 721 ggggcaaaac tctggtgatg gccatctatg actttgaccg cttctccaaa catgacatca 781 ttggagaggt aaaggtgcct atgaacacag tggacctcgg ccagcccatt gaggagtgga 841 gagacctgca aggcggggaa aaggaggagc cggagaagct gggcgacatc tgcacctccc 901 tgcgctatgt gcccacggcc gggaagctca ctgtctgcat cctggaggct aagaacctca 961 agaagatgga cgtgggcggc ctttcagacc cgtacgtgaa gatccacctg atgcagaatg 1021 gcaagaggct caagaagaag aagacaaccg tgaagaagaa gaccctgaac ccatacttca 1081 acgagtcctt cagctttgag atccccttcg agcagattca gaaagtccag gtagtggtca 1141 ccgtgctgga ctatgacaag ctgggcaaga acgaagccat aggcaagatc ttcgtgggca 1201 gcaatgccac gggcacagag ctgcggcact ggtccgacat gctggccaac ccccggaggc 1261 ccatcgccca gtggcactcg ctcaagcctg aggaggaggt ggatgcactc ctgggcaaga 1321 acaagttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1381 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1441 acctctggat ta