Transcript: Mouse XM_006529322.1

PREDICTED: Mus musculus synaptotagmin II (Syt2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Syt2 (20980)
Length:
7026
CDS:
290..1558

Additional Resources:

NCBI RefSeq record:
XM_006529322.1
NBCI Gene record:
Syt2 (20980)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006529322.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000093232 ACCGTGCTAGACTACGACAAA pLKO.1 1370 CDS 100% 4.950 6.930 N Syt2 n/a
2 TRCN0000382427 TTTGAACATCTCTCTTCATTT pLKO_005 2052 3UTR 100% 13.200 9.240 N Syt2 n/a
3 TRCN0000381205 CCCTGGTGATGGCAATCTATG pLKO_005 960 CDS 100% 10.800 7.560 N Syt2 n/a
4 TRCN0000220101 GAAGAAACAGAAGTCCCATTA pLKO.1 2405 3UTR 100% 10.800 7.560 N SYT2 n/a
5 TRCN0000379589 TGAACATGAAGGACATGAAAG pLKO_005 615 CDS 100% 10.800 7.560 N SYT2 n/a
6 TRCN0000380161 TGTTGCTTCTGCATCTGTAAG pLKO_005 533 CDS 100% 10.800 7.560 N Syt2 n/a
7 TRCN0000093230 CCCAGCCTTCAATGAGACATT pLKO.1 901 CDS 100% 4.950 3.465 N Syt2 n/a
8 TRCN0000093231 GTGATGGCAATCTATGACTTT pLKO.1 965 CDS 100% 4.950 3.465 N Syt2 n/a
9 TRCN0000093229 CCTAGAATTAAGCTCCTGGAT pLKO.1 2435 3UTR 100% 2.640 1.848 N Syt2 n/a
10 TRCN0000381185 CCAGAGAACCTGGGCAAATTG pLKO_005 707 CDS 100% 13.200 7.920 N Syt2 n/a
11 TRCN0000093233 CCCAGACAAGAAGAAGAAATA pLKO.1 850 CDS 100% 13.200 7.920 N Syt2 n/a
12 TRCN0000380962 TCAACGAGTCCTTCAGCTTTG pLKO_005 1308 CDS 100% 6.000 3.600 N SYT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006529322.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04832 pDONR223 100% 88.7% 97.6% None (many diffs) n/a
2 ccsbBroad304_04832 pLX_304 0% 88.7% 97.6% V5 (many diffs) n/a
3 TRCN0000466556 GAATAGCACAAAAGCATATTCCAT pLX_317 29.6% 88.7% 97.6% V5 (many diffs) n/a
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