Transcript: Human XM_017000311.2

PREDICTED: Homo sapiens synaptotagmin 2 (SYT2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SYT2 (127833)
Length:
7737
CDS:
308..1576

Additional Resources:

NCBI RefSeq record:
XM_017000311.2
NBCI Gene record:
SYT2 (127833)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017000311.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000356301 CTCTGGTGATGGCCATCTATG pLKO_005 978 CDS 100% 10.800 8.640 N SYT2 n/a
2 TRCN0000356300 CAAACTGAAGGAGAAGTTATT pLKO_005 442 CDS 100% 13.200 9.240 N SYT2 n/a
3 TRCN0000356302 CAAGATCCAGAGCTATCAATA pLKO_005 1617 3UTR 100% 13.200 9.240 N SYT2 n/a
4 TRCN0000356304 GCTTCTGCATCTGCAAGAAAT pLKO_005 546 CDS 100% 13.200 9.240 N SYT2 n/a
5 TRCN0000220101 GAAGAAACAGAAGTCCCATTA pLKO.1 2501 3UTR 100% 10.800 7.560 N SYT2 n/a
6 TRCN0000356303 GACATCATTGGAGAGGTAAAG pLKO_005 1022 CDS 100% 10.800 7.560 N SYT2 n/a
7 TRCN0000380962 TCAACGAGTCCTTCAGCTTTG pLKO_005 1326 CDS 100% 6.000 4.200 N SYT2 n/a
8 TRCN0000220102 GAAGAAGACCCTGAACCCATA pLKO.1 1303 CDS 100% 4.050 2.835 N SYT2 n/a
9 TRCN0000220100 CGGGAAGCTCACTGTCTGCAT pLKO.1 1168 CDS 100% 0.880 0.616 N SYT2 n/a
10 TRCN0000010654 GATGACGACGACGCAGAGACA pLKO.1 662 CDS 100% 0.880 0.616 N SYT2 n/a
11 TRCN0000379589 TGAACATGAAGGACATGAAAG pLKO_005 624 CDS 100% 10.800 6.480 N SYT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017000311.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04832 pDONR223 100% 99.2% 99.2% None 343_351delCAGCTCCCA n/a
2 ccsbBroad304_04832 pLX_304 0% 99.2% 99.2% V5 343_351delCAGCTCCCA n/a
3 TRCN0000466556 GAATAGCACAAAAGCATATTCCAT pLX_317 29.6% 99.2% 99.2% V5 343_351delCAGCTCCCA n/a
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