Construct: ORF ccsbBroad304_04893
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007846.1_s304c1, BRDN0000403963
- Derived from:
- ccsbBroadEn_04893
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDCD2 (134492)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04893
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134492 | NUDCD2 | NudC domain containing 2 | NM_145266.6 | 100% | 100% | |
| 2 | human | 134492 | NUDCD2 | NudC domain containing 2 | NM_001329991.2 | 65.4% | 61.1% | (many diffs) |
| 3 | human | 134492 | NUDCD2 | NudC domain containing 2 | NR_138427.2 | 5.7% | 1_52del;240_409del;694_8137del | |
| 4 | human | 134492 | NUDCD2 | NudC domain containing 2 | NR_138426.2 | 5.5% | 1_52del;240_774del;1059_8502del | |
| 5 | mouse | 52653 | Nudcd2 | NudC domain containing 2 | NM_026023.5 | 90.2% | 99.3% | (many diffs) |
| 6 | mouse | 52653 | Nudcd2 | NudC domain containing 2 | NM_001290697.1 | 76.4% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggccccgttt gaggagcgga gtggggtggt accgtgcggg accccgtggg 121 gccagtggta ccagaccttg gaggaggtgt tcattgaagt tcaggtgccg ccaggcacgc 181 gcgcccagga tatccagtgc ggcctccaga gccggcatgt ggcgctgtcg gtgggcggcc 241 gcgagatcct caagggcaaa ctctttgatt ctacaatagc tgatgaggga acatggactt 301 tggaggacag aaaaatggtt cgtattgttc ttacaaagac aaagagagat gcagcaaatt 361 gttggacttc tctactagaa tctgaatatg cagcggatcc ttgggtgcaa gaccaaatgc 421 agagaaagct tacattagag agattccaaa aagaaaatcc tggttttgac ttcagtggag 481 cagaaatctc aggaaactac actaaaggtg gaccagattt ctcaaacctt gagaaatacc 541 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 601 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 661 tta