Construct: ORF ccsbBroad304_04989
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012538.1_s304c1, BRDN0000399276
- Derived from:
- ccsbBroadEn_04989
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSG1L (146395)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04989
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146395 | GSG1L | GSG1 like | NM_144675.2 | 100% | 100% | |
| 2 | human | 146395 | GSG1L | GSG1 like | NM_001323901.2 | 58.8% | 55% | (many diffs) |
| 3 | human | 146395 | GSG1L | GSG1 like | NM_001109763.2 | 53.1% | 53.1% | 1_465del |
| 4 | human | 146395 | GSG1L | GSG1 like | NM_001323900.2 | 50.4% | 50.4% | 1_465del;830_883del |
| 5 | human | 146395 | GSG1L | GSG1 like | XM_011545750.2 | 47.3% | 42.2% | (many diffs) |
| 6 | human | 146395 | GSG1L | GSG1 like | XM_011545751.2 | 31.1% | 26.6% | (many diffs) |
| 7 | mouse | 269994 | Gsg1l | GSG1-like | XM_006507899.3 | 88.5% | 93.2% | (many diffs) |
| 8 | mouse | 269994 | Gsg1l | GSG1-like | NM_001101488.1 | 48.6% | 51.2% | (many diffs) |
| 9 | mouse | 269994 | Gsg1l | GSG1-like | XM_006507898.3 | 33% | 33.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg tctcgagctc ttccactcca gcaatgtcat cgacgggctc aagctcaatg 121 ccttcgcggc tgtcttcacg gtgctctcag gcctcctggg aatggtcgcc cacatgatgt 181 acacgcaggt gttccaggtc accgtgagcc tcggtcctga ggactggaga ccccattcct 241 gggactacgg gtggtccttc tgcctggcgt ggggctcctt tacctgctgc atggcagcct 301 ctgtcaccac gctcaactcc tacaccaaga cggtcattga gttccggcac aagcgcaagg 361 tctttgagca gggctaccgg gaagagccga ccttcataga ccctgaggcc atcaagtact 421 tccgggagag gatggagaag agggacggga gcgaggagga ctttcactta gactgccgcc 481 acgagagata ccctgcccga caccagccac acatggcgga ttcctggccc cggagctccg 541 cacaggaagc accagagctg aaccgacagt gctgggtctt ggggcactgg gtgtgcccaa 601 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 661 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta