Construct: ORF ccsbBroad304_05068
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000790.1_s304c1, BRDN0000411265
- Derived from:
- ccsbBroadEn_05068
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM53A (152877)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05068
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152877 | FAM53A | family with sequence simila... | NM_001013622.3 | 100% | 100% | |
| 2 | human | 152877 | FAM53A | family with sequence simila... | NM_001174070.2 | 100% | 100% | |
| 3 | human | 152877 | FAM53A | family with sequence simila... | XM_005247948.3 | 100% | 100% | |
| 4 | human | 152877 | FAM53A | family with sequence simila... | XM_011513402.2 | 100% | 100% | |
| 5 | human | 152877 | FAM53A | family with sequence simila... | XM_011513403.1 | 78.2% | 74.7% | (many diffs) |
| 6 | human | 152877 | FAM53A | family with sequence simila... | XM_024453912.1 | 78.2% | 74.7% | (many diffs) |
| 7 | human | 152877 | FAM53A | family with sequence simila... | XM_011513404.2 | 76.3% | 74.1% | (many diffs) |
| 8 | human | 152877 | FAM53A | family with sequence simila... | XM_011513405.1 | 74.5% | 74.3% | (many diffs) |
| 9 | human | 152877 | FAM53A | family with sequence simila... | NM_001297435.1 | 68.6% | 76.4% | 881_1034del;1080_1081ins268 |
| 10 | human | 152877 | FAM53A | family with sequence simila... | XM_017007802.1 | 68.6% | 76.4% | 881_1034del;1080_1081ins268 |
| 11 | human | 152877 | FAM53A | family with sequence simila... | XR_924913.3 | 42.6% | 1_895del;2090_2800del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 65
- ORF end:
- 1259
- ORF length:
- 1194
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tggcatggtc acactcatca ctgagaagct gcagagccag agcctggacg acctcacctg 121 caaggcggag gctggcccgt tgcagtattc tgcggaaacc ctgaacaaga gcggtcgtct 181 gttccctttg gagctcaacg accagagtcc ctggaaggtc ttcagtggag gaccgcccgt 241 cagaagccag gcagccacgg gccctgattt ctccttcctg ccgggcctgt ctgctgccgc 301 tcacaccatg ggtcttcagt ggcagccaca gtccccgcgc ccaggcgcag gcctgggtgc 361 agccagcacc gtggacccca gtgaaagcac aggctcgtcc acggccccac cgaccaagcg 421 gcattgccgg tccttgtcag aacccgagga gcttgtgcgc tgccggtccc cctggcgccc 481 cggcagctcc aaggtctgga ctccagtctc caagaggcgg tgcgacagcg gcgggagtgc 541 cacgcggcag ggaagccccg gcgccgtcct gccgaggagt gctgtgtggt cgaccggtcc 601 cacctcgccc gccacgcccc ggccgtcctc cgccagcggc ggcttcgtgg acagcagcga 661 gggcagtgcg ggctcaggcc cgctctggtg ttccgcggag tcctgcttgc cctccacgag 721 gcgccgcccg tccctctcac aggagcgact cgcgggtgcg ggcactcccc tgccctgggc 781 cagcagcagc cccacgtcca cgcctgcgct gggcgggcgc cgtgggctgc tccggtgccg 841 ctcacagcct tgcgtgctca gtgggaagag gagccggcgc aaacggaggc gtgaggagga 901 cgccaggtgg acacgcccat ccttggactt cctgaaaatg acccagactt taaaaaattc 961 aaaaagcctt tgctccctca attacgaaga tgacgatgag gatgacaccc cagtgaagac 1021 ggttctgtcc tccccatgtg actcccgggg cctccctggc atcaccatgc ctggctgcag 1081 ccagaggggc ctcaggacca gccctgtcca ccccaacctg tgggcctcta gggagtcggt 1141 gaccagtgat ggctcccgca ggagcagcgg ggacccccgt gatggggaca gtgtcgggga 1201 ggagggcgtc ttcccccggg cccgctggga gctggacctg gagcagatcg agaacaactg 1261 cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga 1321 ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg 1381 gatta