Construct: ORF ccsbBroad304_05084

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009678.1_s304c1, BRDN0000401970
Derived from:
ccsbBroadEn_05084
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IQUB (154865)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05084
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 154865 IQUB IQ motif and ubiquitin doma... NM_001282855.1 100% 100%
2 human 154865 IQUB IQ motif and ubiquitin doma... NM_001321293.1 100% 100%
3 human 154865 IQUB IQ motif and ubiquitin doma... NM_178827.5 100% 100%
4 human 154865 IQUB IQ motif and ubiquitin doma... XM_005250161.3 100% 100%
5 human 154865 IQUB IQ motif and ubiquitin doma... XM_011515833.2 100% 100%
6 human 154865 IQUB IQ motif and ubiquitin doma... XM_011515834.3 100% 100%
7 human 154865 IQUB IQ motif and ubiquitin doma... XM_017011771.1 100% 100%
8 human 154865 IQUB IQ motif and ubiquitin doma... XM_005250162.5 94.7% 92% (many diffs)
9 human 154865 IQUB IQ motif and ubiquitin doma... NR_104244.1 71.9% 1_164del;1921_2116del;2734_3298del
10 human 154865 IQUB IQ motif and ubiquitin doma... NR_104245.1 70.5% 1_177del;1411_1412ins176;2375_2939del
11 human 154865 IQUB IQ motif and ubiquitin doma... XM_017011772.2 64.7% 64.7% 397_398ins837
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2442
ORF length:
2373
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtctaatcaa caggagaagt atgaagctca gaatatagtc aattcaacag
121aagagagtga tgatgctttt gatactgtca ctattccagt tccctcagaa gagcctcaag
181agtcagatca aactgaagag catgaatctg gaatagaaca attcagtgag agccatgcaa
241tacatgttga ggagcagagt gaccaaagct tttcaagcct ggaaccagac aatgaacaac
301tcatggaaga ggttatatca ccaagacaag tttcatatac tccgcaacat catgaaaagc
361aatatgcaat gcagaggcca aatgatgata gtttggcatt tctggataaa ataaagtctg
421taaaggaatc tttgcaagaa tcagtggaag attctctagc aacagtaaaa gttgtactta
481ttccagtggg ccaggaaatt gtaatacctt ttaaggttga taccattctt aaatatctta
541aggaccattt ttcacactta ttaggtatcc cacattctgt actgcagata agatactcag
601gaaaaattct taaaaataat gagactctag tacaacatgg agttaagcca caggaaattg
661tacaagtgga aatcttttct acaaatccag atctgtatcc agtcagaaga atagatggat
721taactgatgt ctctcaaatc ataactgtca ctgtccaaac tggacttgat caataccagc
781aggtacctgt tgagattgtc aaatctgact ttcacaaacc atttcttggt ggattcagac
841ataaagtaac aggagtagag tatcacaatg ctggaacaca aactgtacct aaaaggattc
901ccgaaagact cagtatattt tgtagggata cgcagacagt ttttcagaaa aaaaatctcc
961aacaaactac aaatacaaca tccacacaga tgactaacat tggtgtatat gtatcaaata
1021tgactgataa actggtaaca ccaggaaagt atttttcagc agcagaatac catgctcaaa
1081gactaaaggc ggtgatagtg atacagactt actacaggca atggcatgct aaaatcttcg
1141tagagaattt aagaagacag aaaagcttaa gactggaatg ggaaacacag caagaactaa
1201ggaagataag agaaaaagaa gaatggataa aattggacta tcaccggagg cataatccta
1261aaacaaatga agattttgaa tttctttata atgcattaga attctggcgg caagaagaac
1321ttacacgtat taaccaatct tttactggag ctgaaaggaa agctgctctg tgtgaacttc
1381tggaaaaaga gactcagata attgcttcca ttgggagaca tagatacatt gcttatatgg
1441caaatcagga agcagcaata caagcttttt tggataagtg ttcagctcca aaaatatgga
1501gaacccctaa tggcaaaaca attgagatgg atacgcagtt caccatcaga gccagagagc
1561tgcaaaatat ctataagtgc attatgctga aaaatatctc ccaagatgag aggctggatg
1621tgctgttaac tcttaaacac actgtaaagg aacatgaatg taaactaact caggaaattc
1681tagaattgat tgacagagag gttgacctta tgatgagagg agtcaaacat cataaccttg
1741aaggactcag aaaaagaatt gcgacactct tttttcatta tatcaaaaca cctctgttta
1801atcctgaagt tgcaaaatac cttaaggtcc ctcaagaccc attgaaattt tataagaaga
1861tttacttttg ccacagttgc cagctttatt tgccttctac agaattttct gtatcatcca
1921cctcacgccg catataccgg tgtcgtaact gcattaacct tcagaatgag gctcaaaaac
1981gagaatcatt tttgaagtac aaatgtttac ttcaacagct ctactataca gaagctgatt
2041atgaagatga ttctaaaatt gctttcttga tgcagctaca agacattcag tacctgacag
2101agaacatctg ggcgtcccag tcagtcctca gtgcttgcga caatctcagt gatctggtca
2161tggtcagatg gaataaatcc ctggagtggt ccccctggaa ctgcattctt cttaccaaag
2221atgaagcagc tgctcatctc aagctaacaa gtattgaaga gggatatgaa cgctcattta
2281ttcacaagat caaacacaaa catatcctgg ctaagaacta tttttctcag gttccagtgc
2341tggcttcatt tatacttgat gatggtgaaa ttgatgagat cagatggaag tatcactcag
2401acacaacacc taagattata gaatcccaga ggcctcctca tttgccaact ttcttgtaca
2461aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2521ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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