Construct: ORF ccsbBroad304_05093
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003033.1_s304c1, BRDN0000462091
- Derived from:
- ccsbBroadEn_05093
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASEF (158158)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05093
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158158 | RASEF | RAS and EF-hand domain cont... | NM_152573.4 | 100% | 100% | |
| 2 | human | 158158 | RASEF | RAS and EF-hand domain cont... | XM_005251730.4 | 96.8% | 96.8% | 959_960ins69 |
| 3 | human | 158158 | RASEF | RAS and EF-hand domain cont... | XM_005251731.4 | 82% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2289
- ORF length:
- 2220
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggcggat ggggacggag aggagctggc ccggctgcgc tcagtcttcg 121 ccgcctgcga cgcgaaccgc tcggggcgcc tggagcgcga ggagttccgg gcactgtgca 181 cggagctgcg ggtgcggccg gccgacgccg aggcagtatt ccagcggctg gacgccgacc 241 gtgacggcgc catcaccttc caggagttcg cgcgtggctt cctcgggtcc ctccgcgggg 301 ggcggcgccg ggactggggt cctctggatc ccgcgcccgc cgtgtctgag gcggggccgg 361 agacacacga cagcgaggag gacgaaggcg acgaggacgc ggcggcggcg ctggccacct 421 cgtgcggccc ggcgagtccc ggccgggctt ggcaggattt ccaggcgcga cttggggacg 481 aagccaagtt cattcccaga gaagagcaag ttagtacctt gtaccaaaac atcaaccttg 541 tggagccaag attaattcag ccatatgaac atgttataaa gaacttcatc cgtgagatca 601 gacttcaaag cacagaaatg gaaaatttgg ccattgcggt gaagagagcc caggacaagg 661 cagctatgca gttgagtgag ttggaagagg aaatggatca gaggattcag gctgcagaac 721 ataagacacg gaaagacgaa aaacgcaaag ctgaggaagc cctcagtgac ctcagacgtc 781 agtatgaaac tgaagtagga gatctgcagg tgaccattaa aaagctaaga aagctcgaag 841 aacaatcaaa acgcgtaagt caaaaggaag atgtggctgc attgaaaaaa caaatttatg 901 atttatcaat ggaaaaccag aaagttaaga aagacctttt agaagcacag acaaacatag 961 cctttcttca gagtgagtta gatgctttga aaagtgatta tgctgatcag agtctgaata 1021 ctgaaaggga tctggaaata atccgagcat acacagaaga tcgaaatagt cttgagaggc 1081 aaattgaaat actccaaaca gctaaccgga agctacatga cagtaatgat ggccttagaa 1141 gtgcccttga aaacagttat agcaagttca acagatcttt gcatataaat aatatctcac 1201 cagggaatac aatttctaga agcagtccca aattcattgg tcattcccct caacctctag 1261 gctatgacag gtcatcccgc tcttcctatg tggatgagga ctgtgactcc ctggccctct 1321 gtgatcctct gcagaggaca aattgtgaag ttgacagcct gcctgaaagc tgcttcgaca 1381 gcggcttgtc taccttgaga gatcccaatg agtatgactc agaagtggaa tacaagcacc 1441 agaggggatt tcagaggtca cacggggtgc aggagagctt tggaggtgat gcttcagaca 1501 cagatgttcc tgacataagg gatgaagaga catttggttt agaagatgtg gcttccgtct 1561 tagactggaa gccccaaggg tctgttagtg aaggcagcat tgttagttca tcaagaaagc 1621 ccatctcagc actctcgccc cagacagacc tggtagatga caacgctaaa tcttttagct 1681 cacagaaggc ttacaagatt gtacttgctg gggacgctgc agtggggaag tctagtttcc 1741 tcatgagact ttgcaagaat gaatttcgag aaaatataag cgccaccctg ggagttgatt 1801 tccaaatgaa aaccctcatt gtggatggag aacgaacagt tctgcagctc tgggatacag 1861 ctggtcagga gagattcaga agtattgcca agtcttactt cagaaaggca gatggtgttt 1921 tgctgctgta tgatgttaca tgtgagaaaa gctttcttaa catacgagaa tgggtagata 1981 tgattgagga tgcagcccat gagactgttc ccattatgct ggtaggaaac aaggctgaca 2041 ttcgtgacac tgctgctaca gagggacaaa aatgtgtccc agggcacttt ggagagaaac 2101 tggccatgac gtatggggca ttattctgtg aaacaagtgc caaagatggt tctaacatag 2161 tggaggctgt tctgcacctt gctcgagaag tgaaaaagag aactgacaag gatgacagca 2221 gatccattac caatctaacc gggaccaatt ccaaaaagtc accacagatg aagaattgtt 2281 gcaatggctt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 2341 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 2401 tcaacctctg gatta