Construct: ORF ccsbBroad304_05167
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006550.1_s304c1, BRDN0000402221
- Derived from:
- ccsbBroadEn_05167
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCAMP5 (192683)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05167
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 192683 | SCAMP5 | secretory carrier membrane ... | NM_001178111.2 | 100% | 100% | |
| 2 | human | 192683 | SCAMP5 | secretory carrier membrane ... | NM_001178112.2 | 100% | 100% | |
| 3 | human | 192683 | SCAMP5 | secretory carrier membrane ... | NM_138967.4 | 100% | 100% | |
| 4 | human | 192683 | SCAMP5 | secretory carrier membrane ... | XM_006720421.1 | 100% | 100% | |
| 5 | human | 192683 | SCAMP5 | secretory carrier membrane ... | XM_006720420.1 | 92.8% | 92.8% | 1_54del |
| 6 | human | 192683 | SCAMP5 | secretory carrier membrane ... | XM_006720422.3 | 69.7% | 58.4% | 0_1ins56;80_81ins157 |
| 7 | human | 192683 | SCAMP5 | secretory carrier membrane ... | XM_017021989.1 | 69.7% | 58.4% | 0_1ins56;80_81ins157 |
| 8 | human | 192683 | SCAMP5 | secretory carrier membrane ... | NR_033660.2 | 16.2% | 1_159del;295_296ins157;708_3222del | |
| 9 | mouse | 56807 | Scamp5 | secretory carrier membrane ... | NM_001301634.1 | 92.7% | 98.7% | (many diffs) |
| 10 | mouse | 56807 | Scamp5 | secretory carrier membrane ... | NM_020270.3 | 92.7% | 98.7% | (many diffs) |
| 11 | mouse | 56807 | Scamp5 | secretory carrier membrane ... | XM_006511320.3 | 92.7% | 98.7% | (many diffs) |
| 12 | mouse | 56807 | Scamp5 | secretory carrier membrane ... | XM_017313495.1 | 92.7% | 98.7% | (many diffs) |
| 13 | mouse | 56807 | Scamp5 | secretory carrier membrane ... | NM_001301635.1 | 63.1% | 56% | (many diffs) |
| 14 | mouse | 56807 | Scamp5 | secretory carrier membrane ... | XR_001778947.1 | 19.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agagaaagtg aacaacttcc caccattgcc caaattcatc ccgctgaagc 121 catgtttcta ccaagacttc gaggcagata ttcctcccca gcatgtcagc atgaccaagc 181 gcctctacta cctctggatg ttgaacagcg tcacgctggc cgtgaacctg gtgggctgtc 241 tcgcgtggct gatcggaggc gggggagcca ccaactttgg cctcgccttt ctctggctca 301 tcctcttcac accctgctcc tacgtctgct ggtttcggcc catttacaag gccttcaaga 361 ctgacagctc cttcagtttc atggcattct tctttacctt catggctcag ttggtcatca 421 gcatcatcca ggccgtgggc atcccaggct ggggcgtctg cggctggatt gctaccatct 481 ccttcttcgg aacgaacatt ggctcggcgg tggtgatgct aattcccact gtcatgttca 541 cagtgatggc cgtcttttcc ttcatcgccc tcagcatggt tcataaattt taccggggaa 601 gtggggggag tttcagcaaa gctcaggagg agtggaccac aggggcctgg aagaatccac 661 atgtgcagca ggcagcccag aacgcagcca tgggggcagc ccagggtgcc atgaatcagc 721 ctcagactca gtattccgcc acccccaatt acacgtactc caatgagatg tgcccaactt 781 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 841 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta