Construct: ORF ccsbBroad304_05200
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003542.1_s304c1, BRDN0000403354
- Derived from:
- ccsbBroadEn_05200
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMIM14 (201895)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05200
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 201895 | SMIM14 | small integral membrane pro... | NM_001317896.2 | 100% | 100% | |
| 2 | human | 201895 | SMIM14 | small integral membrane pro... | NM_001317897.2 | 100% | 100% | |
| 3 | human | 201895 | SMIM14 | small integral membrane pro... | NM_174921.3 | 100% | 100% | |
| 4 | mouse | 68552 | Smim14 | small integral membrane pro... | NM_133697.3 | 82.4% | 89.8% | (many diffs) |
| 5 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504083.2 | 82.4% | 89.8% | (many diffs) |
| 6 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504084.3 | 82.4% | 89.8% | (many diffs) |
| 7 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504085.3 | 82.4% | 89.8% | (many diffs) |
| 8 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504086.3 | 82.4% | 89.8% | (many diffs) |
| 9 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504087.3 | 82.4% | 89.8% | (many diffs) |
| 10 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504088.3 | 82.4% | 89.8% | (many diffs) |
| 11 | mouse | 68552 | Smim14 | small integral membrane pro... | XM_006504089.2 | 82.4% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 363
- ORF length:
- 297
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agaaggtgga tttgatccct gtgaatgtgt ttgctctcat gaacatgcaa 121 tgagaagact gatcaatctg ttacggcagt cccagtccta ctgcacagac acagagtgtc 181 ttcaggaatt accgggaccc tctggtgata atggcatcag tgttacaatg atcttggtag 241 cctggatggt tattgcattg atcttgttct tactgagacc tcctaatcta agaggatcca 301 gcctacctgg aaagccaacc agtcctcata atggacaaga tccaccagct cctcctgtgg 361 actaccnaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 421 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 481 tctggatta