Construct: ORF ccsbBroad304_05267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010376.1_s304c1, BRDN0000406773
- Derived from:
- ccsbBroadEn_05267
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCZ1B (221960)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05267
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221960 | CCZ1B | CCZ1 homolog B, vacuolar pr... | NM_198097.3 | 100% | 100% | |
| 2 | human | 51622 | CCZ1 | CCZ1 homolog, vacuolar prot... | NM_015622.6 | 99.8% | 100% | 759T>C;1104C>T |
| 3 | mouse | 231874 | Ccz1 | CCZ1 vacuolar protein traff... | NM_177682.3 | 88.5% | 95.8% | (many diffs) |
| 4 | mouse | 231874 | Ccz1 | CCZ1 vacuolar protein traff... | XM_017320876.1 | 62.4% | 67.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1512
- ORF length:
- 1446
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcagcggcg gccggggccg ggagcgggcc ctgggcggcc caggagaagc 121 agttcccgcc ggcgctgctg agtttcttca tctacaaccc gcgcttcggg ccgcgcgaag 181 gacaggagga aaataagatt ttattttatc atccaaatga ggtagaaaag aatgagaaga 241 ttagaaatgt cggattgtgt gaagctattg tacagtttac aaggacattt agcccatcaa 301 aacctgcaaa atctttacat acacagaaga acagacagtt cttcaatgaa ccagaagaaa 361 atttctggat ggtcatggtt gttcggaatc ctataattga aaaacagagt aaagatggaa 421 aaccagttat tgaatatcaa gaggaggagt tgttggacaa ggtttatagc tcggtgctgc 481 ggcagtgcta cagcatgtac aagcttttta atggtacatt tctgaaagcc atggaagacg 541 gaggcgtcaa gcttctgaaa gaaagattag agaaattctt ccatcggtat ttgcaaacgc 601 tacatttgca gtcatgtgac ctacttgaca tttttggtgg aatcagcttc ttcccgttgg 661 ataaaatgac ttatttgaaa atccagtcct ttattaatag aatggaggaa agcctgaata 721 tagtcaaata cactgctttt ctctataacg atcagctcat ctggagtgga ttagaacaag 781 atgacatgag aattttatac aaatacctta ccacctccct tttcccaagg cacatcgaac 841 ctgagttagc aggaagggat tctccaataa gagcagaaat gccaggaaat cttcaacact 901 atggaagatt tcttaccgga cccttgaacc tcaatgatcc agatgcaaaa tgcagattcc 961 ccaaaatttt tgtaaataca gatgacactt atgaagagct ccatttaatc gtttataagg 1021 ccatgagtgc ggctgtgtgc tttatgatcg acgcctctgt ccacccaacg ttggattttt 1081 gccgaagact ggacagcatc gttgggcccc agctcacagt gctggcctct gacatctgtg 1141 aacagtttaa catcaacaag aggatgtctg ggtctgagaa agaaccccag tttaagttta 1201 tctacttcaa ccacatgaat ctcgccgaga agagcacagt tcacatgagg aaaacgccca 1261 gcgtgtcgct cacttccgtg cacccggatt taatgaagat tctcggtgac atcaacagtg 1321 actttaccag agtggatgaa gatgaggaga tcattgtgaa ggccatgagt gattactggg 1381 ttgttggaaa gaagtctgat cggcgggagc tctatgttat tttgaatcaa aaaaatgcaa 1441 acctgattga agtaaatgaa gaggtcaaga aactttgtgc aacgcagttc aacaacatct 1501 tcttcttgga ttgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1561 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1621 caatcaacct ctggatta