Construct: ORF ccsbBroad304_05271
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007472.1_s304c1, BRDN0000401091
- Derived from:
- ccsbBroadEn_05271
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LNX2 (222484)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05271
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222484 | LNX2 | ligand of numb-protein X 2 | NM_153371.4 | 100% | 100% | |
| 2 | human | 222484 | LNX2 | ligand of numb-protein X 2 | XM_017020434.1 | 100% | 100% | |
| 3 | human | 222484 | LNX2 | ligand of numb-protein X 2 | XM_011534995.2 | 82.1% | 82.1% | 850_851ins369 |
| 4 | mouse | 140887 | Lnx2 | ligand of numb-protein X 2 | NM_080795.4 | 80.3% | 89.8% | (many diffs) |
| 5 | mouse | 140887 | Lnx2 | ligand of numb-protein X 2 | XM_006504800.3 | 80.3% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2136
- ORF length:
- 2070
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg aacaacaagt gatgagatgg tgtctgtgga acagacctcc tcctcttctc 121 taaaccccct gtgttttgaa tgtggccaac agcactggac aagagaaaac catttgtaca 181 attaccagaa tgaagtggat gatgacctag tctgccatat ttgccttcaa cctctgctgc 241 agccactaga cacaccctgt ggacatacat tctgctacaa gtgcctcaga aactttttac 301 aagagaaaga tttctgtccg ttggaccgga aaagacttca ttttaagttg tgcaagaagt 361 ctagtattct agttcataaa ctcctagaca aattattagt tttatgtcca ttttcttcag 421 tgtgcaaaga tgtaatgcaa cgttgtgatc tggaggcaca tctcaaaaac agatgtcctg 481 gagcttctca tcggagagtt gccctggaga gaaggaaaac tagtagaact caagcagaga 541 ttgagaatga aaatgggccc actctactag atcctgcagg taccttatct ccagaagcag 601 actgtttggg gacaggcgca gtgcctgtgg agcggcactt gacatcagcg tctctttcca 661 catggagtga ggagcctggc cttgacaacc ctgcctttga ggagagcgct ggagctgaca 721 ccacacaaca gccacttagt ttaccagaag gagaaatcac cacgattgaa attcatcggt 781 ccaatcctta cattcagtta ggaatcagca ttgtgggtgg caacgaaaca cctttgatta 841 acattgtcat ccaggaggtc tatcgggatg gggtcattgc cagagacggg agacttcttg 901 ctggagacca gattcttcag gtcaacaact acaatatcag caatgtgtcc cataactatg 961 cccgagctgt cctttcccag ccctgcaaca cactgcatct tactgtgctt cgagagaggc 1021 gctttggcaa ccgagcacac aaccattctg atagtaactc tccacgagaa gagattttcc 1081 aagtggctct tcataaacgg gactctggtg aacagcttgg cattaaattg gtgcgaagga 1141 cagatgagcc aggggttttt attcttgacc tgttggaagg ggggttggct gcccaggacg 1201 gcaggctaag cagcaatgac cgagtgctgg ccatcaatgg gcacgacctg aagtatggaa 1261 ctccggagct tgctgcccag attattcagg ccagtggaga gagagtgaat ttaacaattg 1321 ctagaccagg gaaaccccag cctggtaaca ccattagaga agcaggaaat catagcagca 1381 gcagccagca ccacacacca ccaccgtatt atagcagacc aagctcacat aaggatctta 1441 ctcagtgtgt tacatgccaa gaaaaacaca ttactgtaaa gaaggaacca catgaatccc 1501 ttggcatgac cgttgctggg ggcaggggaa gtaagagtgg tgagctgccc atctttgtga 1561 ccagtgtgcc accccatggc tgccttgcac gagatggcag aataaagaga ggtgatgtgt 1621 tgctaaatat caacggcatt gatttgacca atttaagtca cagtgaggca gttgcaatgc 1681 tgaaagccag tgccgcgtcc cctgctgttg cccttaaagc acttgaggtc cagattgttg 1741 aggaggcgac tcagaacgcg gaggagcagc cgagtacttt cagcgaaaat gagtatgatg 1801 ccagttggtc cccatcatgg gtcatgtggc ttgggcttcc cagcacactt catagctgcc 1861 acgatatagt tttacgaaga agttacttgg gaagttgggg ctttagtatc gttggtggat 1921 atgaagagaa ccacaccaat cagccttttt tcattaaaac tattgtcttg ggaactcctg 1981 cttattatga tggaagatta aagtgtggtg acatgattgt ggccgtaaat gggctgtcaa 2041 ccgtgggcat gagccactct gcactagttc ccatgttgaa ggagcagagg aacaaagtca 2101 ctctgaccgt tatttgttgg cctggcagcc ttgtataccc aactttcttg tacaaagtgg 2161 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2221 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta