Construct: ORF ccsbBroad304_05335
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000215.1_s304c1, BRDN0000410997
- Derived from:
- ccsbBroadEn_05335
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SELENOH (280636)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05335
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 280636 | SELENOH | selenoprotein H | NM_001321335.1 | 100% | 100% | |
| 2 | human | 280636 | SELENOH | selenoprotein H | NM_170746.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 195
- ORF length:
- 129
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tccccgcggg aggaagcgta aggctgaggc cgcggtggtc gccgtagccg 121 agaagcgaga gaagctggcg aacggcgggg agggaatgga ggaggcgacc gttgttatcg 181 agcattgcac tagctgacgc gtctatgggc gcaacgccgc ggccctgagc caggcgctgc 241 gcctggaggc cccagagctt ccagtaaagg tgaacccgac gaagccccgg aggggcagct 301 tcgaggtgac gctgctgcgc ccggacggca gcagtgcgga gctctggact gggattaaga 361 aggggccccc acgcaaactc aaattccctg agcctcaaga ggtggtggaa gagttgaaga 421 agtacctgtc gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 481 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 541 caatcaacct ctggatta