Construct: ORF ccsbBroad304_05440
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015299.1_s304c1, BRDN0000460571
- Derived from:
- ccsbBroadEn_05440
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUPD1 (338599)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05440
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338599 | DUPD1 | dual specificity phosphatas... | NM_001003892.2 | 100% | 100% | |
| 2 | human | 338599 | DUPD1 | dual specificity phosphatas... | XM_011539747.2 | 100% | 100% | |
| 3 | human | 338599 | DUPD1 | dual specificity phosphatas... | XM_017016176.1 | 44.4% | 35.5% | (many diffs) |
| 4 | mouse | 435391 | Dupd1 | dual specificity phosphatas... | NM_001013826.2 | 83.7% | 82.2% | (many diffs) |
| 5 | mouse | 435391 | Dupd1 | dual specificity phosphatas... | XM_006519217.3 | 83.7% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 729
- ORF length:
- 660
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gacatctgga gaagtgaaga caagcctcaa gaatgcctac tcatctgcca 121 agaggctgtc gccgaagatg gaggaggaag gggaggagga ggactactgc acccctggag 181 cctttgagct ggagcggctc ttctggaagg gcagtcccca gtacacccac gtcaacgagg 241 tctggcccaa gctctacatt ggcgatgagg cgacggcgct ggaccgctat aggctgcaga 301 aggcggggtt cacgcacgtg ctgaacgcgg cccacggccg ctggaacgtg gacactgggc 361 ccgactacta ccgcgacatg gacatccagt accacggcgt ggaggccgac gacctgccca 421 ccttcgacct cagtgtcttc ttctacccgg cggcagcctt catcgacaga gcgctaagcg 481 acgaccacag taagatcctg gttcactgcg tcatgggccg cagccggtca gccaccctgg 541 tcctggccta cctgatgatc cacaaggaca tgaccctggt ggacgccatc cagcaagtgg 601 ccaagaaccg ctgcgtcctc ccgaaccggg gctttttgaa gcagctccgg gagctggaca 661 agcagctggt gcagcagagg cgacggtccc agcgccagga cggtgaggag gaggatggca 721 gggagctgtt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 781 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 841 tcaacctctg gatta