Construct: ORF ccsbBroad304_05519
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009395.1_s304c1, BRDN0000402147
- Derived from:
- ccsbBroadEn_05519
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NMNAT3 (349565)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05519
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | NM_178177.4 | 100% | 100% | |
| 2 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_011512790.1 | 100% | 100% | |
| 3 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_011512791.2 | 100% | 100% | |
| 4 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006338.1 | 100% | 100% | |
| 5 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | NM_001320511.1 | 85.3% | 85.3% | 1_111del |
| 6 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | NM_001320512.1 | 85.3% | 85.3% | 1_111del |
| 7 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006335.2 | 85.3% | 85.3% | 1_111del |
| 8 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006336.2 | 85.3% | 85.3% | 1_111del |
| 9 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006337.2 | 85.3% | 85.3% | 1_111del |
| 10 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | NM_001200047.2 | 69.8% | 63.2% | (many diffs) |
| 11 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | NM_001320513.1 | 66% | 66% | 0_1ins219 |
| 12 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | NM_001320510.2 | 62.1% | 62.1% | 1_393del |
| 13 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006328.1 | 62.1% | 62.1% | 1_393del |
| 14 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006329.1 | 62.1% | 62.1% | 1_393del |
| 15 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006330.1 | 62.1% | 62.1% | 1_393del |
| 16 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006331.1 | 62.1% | 62.1% | 1_393del |
| 17 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006332.1 | 62.1% | 62.1% | 1_393del |
| 18 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006333.1 | 62.1% | 62.1% | 1_393del |
| 19 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006334.1 | 62.1% | 62.1% | 1_393del |
| 20 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | NM_001363968.1 | 47.1% | 36.1% | (many diffs) |
| 21 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006341.1 | 47.1% | 36.1% | (many diffs) |
| 22 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006342.1 | 47.1% | 36.1% | (many diffs) |
| 23 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006343.1 | 47.1% | 36.1% | (many diffs) |
| 24 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006339.1 | 17.6% | 17.3% | 1_393del;576_579delCTGT;582_582delCins461 |
| 25 | human | 349565 | NMNAT3 | nicotinamide nucleotide ade... | XM_017006340.1 | 17.6% | 17.3% | 1_393del;576_579delCTGT;582_582delCins461 |
| 26 | mouse | 74080 | Nmnat3 | nicotinamide nucleotide ade... | XM_006511505.3 | 30.5% | 28.9% | (many diffs) |
| 27 | mouse | 74080 | Nmnat3 | nicotinamide nucleotide ade... | XM_006511506.3 | 30.5% | 28.9% | (many diffs) |
| 28 | mouse | 74080 | Nmnat3 | nicotinamide nucleotide ade... | XM_006511507.3 | 30.5% | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgta ccaggtcatc cagggtatca tctctcctgt caacgacacc tatgggaaga 121 aagacctcgc agcttctcat caccgagtgg ccatggcccg gctggccctg cagacatccg 181 actggatccg ggtggaccct tgggagagtg agcaggcaca gtggatggag acagtgaagg 241 tgctgaggca tcatcacagc aaactgctca gatctccacc ccagatggaa ggcccagacc 301 atggcaaggc actcttctcg acccctgcag ctgtgcctga gctgaagctt ctctgtgggg 361 cagacgtctt gaagaccttc cagaccccca acctctggaa ggatgcgcac atccaggaaa 421 tagtggagaa gtttggcttg gtgtgcgtgg gccgagtagg tcacgaccca aaaggttaca 481 tcgcagaatc tcccatccta cggatgcacc agcacaacat tcacctggcc aaggagcctg 541 tgcagaatga gatcagtgcc acatacatca ggcgagcctt gggccaaggg cagagcgtaa 601 agtacctgat tcccgatgct gtcatcacgt acatcaagga ccatggcctc tacaccaagg 661 gcagtacctg gaaaggcaaa agcacccaga gcactgaggg caagacaagc tacccaactt 721 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 781 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta