Construct: ORF ccsbBroad304_05568
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006667.1_s304c1, BRDN0000460794
- Derived from:
- ccsbBroadEn_05568
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPDYC (387778)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05568
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387778 | SPDYC | speedy/RINGO cell cycle reg... | NM_001008778.2 | 100% | 100% | |
| 2 | human | 387778 | SPDYC | speedy/RINGO cell cycle reg... | XM_017017708.1 | 93.5% | 93.5% | 0_1ins57 |
| 3 | human | 387778 | SPDYC | speedy/RINGO cell cycle reg... | XM_017017707.1 | 85.8% | 85.8% | 0_1ins57;458_535del |
| 4 | human | 387778 | SPDYC | speedy/RINGO cell cycle reg... | XM_017017706.1 | 78.5% | 78.2% | 0_1ins57;791_958del |
| 5 | human | 387778 | SPDYC | speedy/RINGO cell cycle reg... | XM_017017705.1 | 73% | 72.8% | 0_1ins57;458_535del;869_1036del |
| 6 | human | 387778 | SPDYC | speedy/RINGO cell cycle reg... | XM_017017709.1 | 66.3% | 57.7% | (many diffs) |
| 7 | human | 387778 | SPDYC | speedy/RINGO cell cycle reg... | XM_017017710.1 | 59.7% | 59.7% | 0_1ins57;457_458ins297 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 948
- ORF length:
- 879
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctctgggcc attcctgagc tcgggtcacc ctgccccatc tccatctcct 121 atgagatgag tgactcccaa gaccccacca cttcccctgt agttaccacc caggtagagc 181 tggggggctg cagccggcaa ggtgggggca atgggttcct ccgttttcgc cagcaccagg 241 aggtccaggc cttcctcagc cttctggagg acagttttgt ccaggaattc ctctccaaag 301 acccctgctt ccagatttca gataagtatc tcctggccat ggtgctggtc tacttccagc 361 gcgcccacct gaagctcagc gagtataccc acagcagcct gttcttggcc ctgtaccttg 421 caaacgacat ggaggaggac ctggagggcc ccaaatgtga gatttttcca tgggccctgg 481 gaaaagattg gtgtttacga gtggggaaat tcctgcacca gagggataag ctttgggcac 541 ggatgggttt ccgggctgtt gtgagccgcc agtgctgtga ggaggtcatg gcaaaggagc 601 cattccactg ggcttggact cgggaccggc gcccccacca tggtggggtt cagagggtct 661 gtccacaggt ccctgttcgc cttccccggg gccctggcct ctcgccgccc cactgttccc 721 cctgtggttt gccccagcac tgcagcagcc acttgcttaa gcctgtgtca tccaagtgcc 781 cttctctgac ctctgaatgt catcgccctc cctcccaaaa ttatctctca agggtcaaaa 841 acgcctgggg tggggacttt ctcatcgtct tgcctcccca gatgcaactg gaaccaggca 901 cctactccct ccgcatcttc ccaaagcctc cggcacgccc tgggcacttg ccaactttct 961 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1021 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta